采用 NCI 面板检测东亚结直肠癌患者肿瘤 MSI 的临床病理特征和预后
The clinicopathological features and prognosis of tumor MSI in East Asian colorectal cancer patients using NCI panel.
机构信息
Department of Pathology, Changhai Hospital of Shanghai, 168 Changhai Road, Shanghai 200433, PR China.
Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, PR China.
出版信息
Future Oncol. 2018 Jun;14(14):1355-1364. doi: 10.2217/fon-2017-0662. Epub 2018 Jan 25.
AIM
To compare test results obtained from a PCR assay for the National Cancer Institute (NCI) five loci criteria for detecting microsatellite instability (MSI) with those obtained from immunohistochemistry of mismatch repair and a five-mononucleotide site amplification system in East Asian patients with colorectal cancer.
PATIENTS & METHODS: A total of 245 East Asian patients with colorectal cancer were studied retrospectively at our institution.
RESULTS
The consistency of the NCI panel PCR method compared with detection of mismatch repair protein expression by immunohistochemistry was 0.898. High level MSI (MSI-H) status was correlated with the Tumor, Node, Metastasis stage, tumor location site, metastasis, tumor grade, mucinous histological type and BRAF-type mutations.
CONCLUSION
The NCI panel PCR assay has excellent sensitivity and specificity for detecting MSI in an East Asian population.
目的
比较聚合酶链反应（PCR）检测用于检测微卫星不稳定性（MSI）的国家癌症研究所（NCI）五个基因座标准与免疫组化错配修复和五核苷酸位点扩增系统在东亚结直肠癌患者中的检测结果。
患者和方法
本研究回顾性分析了我院 245 例东亚结直肠癌患者。
结果
NCI 面板 PCR 方法与免疫组化检测错配修复蛋白表达的一致性为 0.898。高水平 MSI（MSI-H）状态与肿瘤、淋巴结、转移分期、肿瘤部位、转移、肿瘤分级、黏液组织学类型和 BRAF 型突变相关。
结论
NCI 面板 PCR 检测法在东亚人群中具有检测 MSI 的高灵敏度和特异性。
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