一种高选择性和高灵敏度的 Tb-乙酰丙酮光探针，用于评估药物和血清样品中的乙酰唑胺。
A highly selective and sensitive Tb-acetylacetone photo probe for the assessment of acetazolamide in pharmaceutical and serum samples.
机构信息
Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Apr 15;195:47-52. doi: 10.1016/j.saa.2018.01.047. Epub 2018 Jan 20.
A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of Acetazolamide in pharmaceutical tablets and serum samples using photo probe Tb-ACAC. The Acetazolamide can remarkably quench the luminescence intensity of Tb-ACAC complex in DMSO at pH 6.8 and λ = 350 nm. The quenching of luminescence intensity of Tb-ACAC complex especially the electrical band at λ = 545 nm is used for the assessment of Acetazolamide in the pharmaceutical tablet and serum samples. The dynamic range found for the determination of Acetazolamide concentration is 4.49 × 10-1.28 × 10 mol L, and the limit of detection (LOD) and limit of quantification (LOQ) are (4.0 × 10 and 1.21 × 10) mol L, respectively.
本文建立了一种新颖、简单、灵敏和选择性的光谱荧光法，使用光探针 Tb-ACAC 测定了片剂和血清样品中的乙酰唑胺。在 pH 6.8 和 λ = 350 nm 下，乙酰唑胺可以显著猝灭 DMSO 中 Tb-ACAC 配合物的发光强度。Tb-ACAC 配合物的发光强度猝灭，特别是在 λ = 545 nm 处的电带，用于评估片剂和血清样品中的乙酰唑胺。测定乙酰唑胺浓度的动态范围为 4.49×10-1.28×10 mol·L-1，检测限（LOD）和定量限（LOQ）分别为（4.0×10 和 1.21×10）mol·L-1。
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