Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Talanta. 2015 Jun 1;138:203-208. doi: 10.1016/j.talanta.2015.02.023. Epub 2015 Feb 20.
It was found that silver nanoparticles (AgNPs) could enhance co-luminescence effect of rare earths ions Tb(3+) and Y(3+). Based on this, a sensitive fluorescence detection method for the determination of dopamine (DA) was proposed. Moreover, the detection limit for DA was very low (down to nM). This is because DA can remarkably enhance the luminescence intensity of the Tb(3+) ion by Y(3+) in the colloidal solution of AgNPs, forming a new co-luminescence system. Furthermore, based on the metal enhanced fluorescence (MEF), AgNPs can sensitize the co-luminescence effect of the complex of Tb(3+)-Y(3+)-DA. In a neutral buffer solution (pH 7.50), the luminescence intensity of the system was linearly related to the concentration of DA in the range of 2.0-100 nM, with a limit of detection as low as 0.57 nM. The proposed method was applied for the determination of DA in dopamine hydrochloride injections and human serum samples with good accuracy and satisfactory recovery.
研究发现，银纳米粒子（AgNPs）可以增强稀土离子Tb(3+)和 Y(3+)的共发光效应。基于此，提出了一种用于测定多巴胺（DA）的灵敏荧光检测方法。此外，DA 的检测限非常低（低至 nM）。这是因为 DA 可以显著增强胶体溶液中 AgNPs 中 Y(3+)离子的 Tb(3+)离子的发光强度，形成新的共发光体系。此外，基于金属增强荧光（MEF），AgNPs 可以敏化 Tb(3+)-Y(3+)-DA 配合物的共发光效应。在中性缓冲溶液（pH 7.50）中，该体系的发光强度在 2.0-100 nM 的范围内与 DA 的浓度呈线性关系，检测限低至 0.57 nM。该方法用于测定盐酸多巴胺注射液和人血清样品中的 DA，具有良好的准确性和令人满意的回收率。
