在磷酸果糖激酶柠檬酸调控减弱的黑曲霉突变体中，锰离子对柠檬酸积累的抑制作用
Inhibition of citric acid accumulation by manganese ions in Aspergillus niger mutants with reduced citrate control of phosphofructokinase.
作者信息
Schreferl G, Kubicek C P, Röhr M
出版信息
J Bacteriol. 1986 Mar;165(3):1019-22. doi: 10.1128/jb.165.3.1019-1022.1986.
Abstract
Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants tolerated a higher intracellular citrate concentration than the parent strain. One mutant (cic-7/3) contained phosphofructokinase activity significantly less sensitive towards citrate than the enzyme from the parent strain. When this mutant was grown under citrate-accumulating conditions, acidogenesis was far less sensitive to inhibition by Mn2+ than in the parent strain. Some of the cic mutants also showed altered citrate inhibition of NADP-specific isocitrate dehydrogenase.
摘要
碳水化合物分解代谢的柠檬酸盐控制降低的黑曲霉突变株（cic突变体）在含有5%（重量/体积）柠檬酸盐的培养基上比亲本菌株生长得更快。这些突变体比亲本菌株能耐受更高的细胞内柠檬酸盐浓度。一个突变体（cic-7/3）所含的磷酸果糖激酶活性对柠檬酸盐的敏感性明显低于亲本菌株的该酶。当这个突变体在柠檬酸盐积累条件下生长时，产酸对Mn2+抑制的敏感性远低于亲本菌株。一些cic突变体还表现出对NADP特异性异柠檬酸脱氢酶的柠檬酸盐抑制作用发生改变。
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