Ohtani Naoko, Suda Koshi, Tsuji Erika, Tanemura Kentaro, Yokota Hiroshi, Inoue Hiroki, Iwano Hidetomo
Laboratory of Veterinary Biochemistry, Department of Bioscience, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan.
J Vet Med Sci. 2018 Mar 30;80(3):536-543. doi: 10.1292/jvms.17-0460. Epub 2018 Jan 25.
Bisphenol A (BPA) is among the better-known endocrine disruptors. BPA is used in various food-contacting materials and is easily eluted into food; as a result, we are exposed to BPA on a daily basis. In adults, BPA is metabolized and eliminated rapidly from the body. However, numerous reports suggest that fetuses and young children are susceptible to BPA. One of the concerning adverse effects of BPA is disruption of behavior, especially anxiety-like behavior. In order to study the mechanism of influences on offspring, it is important to clarify the most vulnerable gestation period. We hypothesized that offspring in late pregnancy would be more susceptible to BPA, because late pregnancy is a critical time for functional brain development. In this study, C57BL/6 mouse fetuses were exposed prenatally by oral dosing of pregnant dams, once daily from gestational day 5.5 to 12.5 (early pregnancy) or 11.5 to 18.5 (late pregnancy), with BPA (0 or 10 mg/kg body weight). Following birth and weaning, the resulting pups were tested using an elevated plus maze at postnatal week 10. The behavior of the offspring was altered by prenatal BPA exposure during late pregnancy but not during early pregnancy. These results indicated that offspring are more vulnerable to exposure to BPA in late pregnancy.
双酚A（BPA）是较为知名的内分泌干扰物之一。BPA被用于各种与食品接触的材料中，且很容易溶出到食品中；因此，我们每天都暴露于BPA。在成年人中，BPA在体内会迅速代谢并消除。然而，大量报告表明，胎儿和幼儿易受BPA影响。BPA令人担忧的不良影响之一是行为紊乱，尤其是类似焦虑的行为。为了研究对后代的影响机制，明确最易受影响的孕期很重要。我们推测妊娠晚期的后代对BPA更敏感，因为妊娠晚期是大脑功能发育的关键时期。在本研究中，从妊娠第5.5天至12.5天（妊娠早期）或11.5天至18.5天（妊娠晚期），通过对怀孕母鼠每日口服给药BPA（0或10毫克/千克体重），使C57BL/6小鼠胎儿在产前暴露于BPA。出生和断奶后，在出生后第10周使用高架十字迷宫对所产幼崽进行测试。产前BPA暴露在妊娠晚期会改变后代的行为，但在妊娠早期则不会。这些结果表明，后代在妊娠晚期更容易受到BPA暴露的影响。
