Guo Shuang, Wang Yuehua, Chou Shurui, Cui Huijun, Li Dongnan, Li Bin
College of Food Science, Shenyang Agricultural University, Liaoning, China.
PLoS One. 2018 Jan 25;13(1):e0191762. doi: 10.1371/journal.pone.0191762. eCollection 2018.
In the present study, the DPPH and ABTS+ radical scavenging activity of eight types of apples decreased (P < 0.05) during the 70-day storage at 4°C. The Fushi (F2) apples from Xin Jiang showed the highest radical scavenging activity. For in vivo study, 40 male Kunming mice (body weight 20-25 g) were selected and randomly assigned to four groups (10 mice per group). The F2 groups (F2S, F2 + sterile saline and F2L, F2 + lipopolysaccharide) were administered with 0.3 mL F2 filtrate via gastric intubation daily for 28 days. The control groups (CS, CON + sterile saline and CL, CON + lipopolysaccharide) were treated with sterile saline at the same volume. At day 29, mice of F2L and CL groups were injected with 100 μg/kg body weight of lipopolysaccharide (LPS) intraperitoneally, while those of F2S and CS groups were injected equal volume of sterile saline. In comparison to the CS group, the CL group showed a decrease (P < 0.05) in serum, liver, and hepatic mitochondrial antioxidant capacity, reduction (P < 0.05) in the expression of hepatic antioxidant-related genes, and an increase (P < 0.05) in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), protein carbonyl (PC), and reactive oxygen species (ROS). In comparison to the CL group, the F2L group showed lower (P < 0.05) levels of serum ALT, AST, and ROS, higher (P < 0.05) level of serum, liver, and hepatic mitochondrial antioxidant capacity, increased mitochondrial membrane potential (MMP), and enhanced (P < 0.05) expression of hepatic antioxidant-related genes. These results suggest that F2 may exert protective effect against LPS-induced oxidative damage by improving the antioxidant capacity.
在本研究中，8种苹果的二苯基苦味酰基自由基（DPPH）和阳离子自由基（ABTS+）清除活性在4℃下储存70天期间下降（P<0.05）。来自新疆的富士（F2）苹果表现出最高的自由基清除活性。在体内研究中，选取40只雄性昆明小鼠（体重20 - 25 g），随机分为四组（每组10只）。F2组（F2S，F2 +无菌生理盐水；F2L，F2 +脂多糖）每天经胃管给予0.3 mL F2滤液，持续28天。对照组（CS，CON +无菌生理盐水；CL，CON +脂多糖）用相同体积的无菌生理盐水处理。在第29天，F2L组和CL组的小鼠腹腔注射100 μg/kg体重的脂多糖（LPS），而F2S组和CS组的小鼠注射等量的无菌生理盐水。与CS组相比，CL组血清、肝脏和肝线粒体抗氧化能力下降（P<0.05），肝脏抗氧化相关基因表达降低（P<0.05），血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、丙二醛（MDA）、蛋白质羰基（PC）和活性氧（ROS）增加（P<0.05）。与CL组相比，F2L组血清ALT、AST和ROS水平较低（P<0.05），血清、肝脏和肝线粒体抗氧化能力较高（P<0.05），线粒体膜电位（MMP）增加，肝脏抗氧化相关基因表达增强（P<0.05）。这些结果表明，F2可能通过提高抗氧化能力对LPS诱导的氧化损伤发挥保护作用。
