研究在花酵母菌株生物膜形成过程中，共价连接细胞壁蛋白（Ccw14p）和酵母糖蛋白（Ygp1p）的作用。
Study of the role of the covalently linked cell wall protein (Ccw14p) and yeast glycoprotein (Ygp1p) within biofilm formation in a flor yeast strain.
机构信息
Department of Microbiology, University of Córdoba, Agrifood Campus of International Excellence ceiA3, 14014 Córdoba, Spain.
Department of Agricultural Science, University of Sassari, Viale Italia 39, 07100 Sassari, Italy.
出版信息
FEMS Yeast Res. 2018 Mar 1;18(2). doi: 10.1093/femsyr/foy005.
Flor yeasts are Saccharomyces cerevisiae strains noted by their ability to create a type of biofilm in the air-liquid interface of some wines, known as 'flor' or 'velum', for which certain proteins play an essential role. Following a proteomic study of a flor yeast strain, we deleted the CCW14 (covalently linked cell wall protein) and YGP1 (yeast glycoprotein) genes-codifying for two cell surface glycoproteins-in a haploid flor yeast strain and we reported that both influence the weight of the biofilm as well as cell adherence (CCW14).
花色酵母是指具有在某些葡萄酒的气-液界面形成生物膜能力的酿酒酵母菌株，这种生物膜被称为“flor”或“velum”，其中某些蛋白质起着至关重要的作用。在对一株花色酵母菌株进行蛋白质组学研究后，我们在一株单倍体花色酵母菌株中删除了编码两种细胞表面糖蛋白的 CCW14（共价连接细胞壁蛋白）和 YGP1（酵母糖蛋白）基因，结果表明这两个基因都影响生物膜的重量和细胞黏附（CCW14）。
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