Instituto Carlos Chagas, FIOCRUZ, Rua Algacyr Munhoz Mader, 3775. CIC, Curitiba, PR, 81350-010, Brazil.
UMR BIPAR, Animal Health Laboratory, ANSES, INRA, ENVA, Maisons Alfort, Cedex, France.
Parasit Vectors. 2018 Jan 25;11(1):62. doi: 10.1186/s13071-018-2648-4.
Control of gene expression is crucial for parasite survival and is the result of a series of processes that are regulated to permit fine-tuning of gene expression in response to biological changes during the life-cycle of apicomplexan parasites. Control of mRNA nuclear export is a key process in eukaryotic cells but is poorly understood in apicomplexan parasites. Here, we review recent knowledge regarding this process with an emphasis on T. gondii. We describe the presence of divergent orthologs and discuss structural and functional differences in export factors between apicomplexans and other eukaryotic lineages. Undoubtedly, the use of the CRISPR/Cas9 system in high throughput screenings associated with the discovery of mRNA nuclear export complexes by proteomic analysis will contribute to identify these divergent factors. Ligand-based or structure-based strategies may be applied to investigate the potential use of these proteins as targets for new antiprotozoal agents.
基因表达的控制对寄生虫的生存至关重要，是一系列受调节的过程的结果，这些过程可以精细地调节基因表达，以响应顶复门寄生虫生命周期中的生物学变化。mRNA 核输出的控制是真核细胞中的一个关键过程，但在顶复门寄生虫中了解甚少。在这里，我们综述了这一过程的最新知识，重点是 T. gondii。我们描述了不同的直系同源物的存在，并讨论了出口因子在结构和功能上的差异在顶复门生物和其他真核生物谱系之间。毫无疑问，CRISPR/Cas9 系统在高通量筛选中的应用，以及通过蛋白质组分析发现 mRNA 核输出复合物，将有助于鉴定这些不同的因子。基于配体或基于结构的策略可能被应用于研究这些蛋白质作为新抗原生动物药物靶点的潜在用途。
