Bermejo Elisabeth, Ruiz-Domínguez María C, Cuaresma María, Vaquero Isabel, Ramos-Merchante Adrian, Vega José M, Vílchez Carlos, Garbayo Inés
Algal Biotechnology Group, University of Huelva, CIDERTA, Marine International Campus of Excellence (CEIMAR), Parque Huelva Empresarial S/N, 21007 Huelva, Spain.
Microencapsulation of Bioactive Compounds Laboratory (LAMICBA), Food Sciences and Nutrition Department, Faculty of Health Sciences, University of Antofagasta, Avda. Universidad de Antofagasta 02800, Antofagasta, Chile.
J Biosci Bioeng. 2018 Jun;125(6):669-675. doi: 10.1016/j.jbiosc.2017.12.025. Epub 2018 Jan 20.
In this study, the effect of abiotic stress on the acidophilic eukaryotic microalga, Coccomyxa onubensis, was analyzed for the production of lutein and PUFAs (polyunsaturated fatty acids). It grows autotrophically at a pH of 2.5. It showed a growth rate of 0.30 d, and produced approximately 122.50 mg·L·d biomass, containing lipids (300.39 mg gdw), lutein (5.30 mg gdw), and β-carotene (1.20 mg gdw). The fatty acid methyl ester (FAME) fraction was 89.70 mg gdw with abundant palmitic acid (28.70%) and linoleic acid (37.80%). The addition of 100 mM NaCl improved the growth rate (0.54 d), biomass productivity (243.75 mg·L·d), and lipids accumulation (416.16 mg gdw). The microalga showed a lutein content of 6.70 mg gdw and FAME fraction of 118.90 mg gdw; 68% of the FAMEs were PUFAs. However, when 200-500 mM salt was added, its growth was inhibited but there was a significant induction of lutein (up to 7.80 mg gdw). Under continuous illumination with PAR (photosynthetically active radiations) +UVA (ultraviolet A, 8.7 W m), C. onubensis showed a growth rate of 0.40 d, and produced 226.3 mg·L·d biomass, containing lipids, (487.26 mg gdw), lutein (7.07 mg gdw), and FAMEs (232.9 mg gdw); 48.4% of the FAME were PUFAs. The illumination with PAR + UVB (ultraviolet B, 0.16 W m) was toxic for cells. These results indicate that C. onubensis biomass is suitable as a supplement for functional foods and/or source of high added value products.
在本研究中，分析了非生物胁迫对嗜酸真核微藻卵形 coccomyxa 的影响，以用于叶黄素和多不饱和脂肪酸（PUFAs）的生产。它在pH值为2.5的条件下自养生长。其生长速率为0.30 d⁻¹，每天产生约122.50 mg·L⁻¹的生物量，其中含有脂质（300.39 mg gdw⁻¹）、叶黄素（5.30 mg gdw⁻¹）和β-胡萝卜素（1.20 mg gdw⁻¹）。脂肪酸甲酯（FAME）含量为89.70 mg gdw⁻¹，富含棕榈酸（28.70%）和亚油酸（37.80%）。添加100 mM NaCl可提高生长速率（0.54 d⁻¹）、生物量生产力（243.75 mg·L⁻¹·d⁻¹）和脂质积累（416.16 mg gdw⁻¹）。该微藻的叶黄素含量为6.70 mg gdw⁻¹，FAME含量为118.90 mg gdw⁻¹；68%的FAME为PUFAs。然而，当添加200 - 500 mM盐时，其生长受到抑制，但叶黄素显著诱导（高达7.80 mg gdw⁻¹）。在光合有效辐射（PAR）+紫外线A（UVA，8.7 W m⁻²）的连续光照下，卵形 coccomyxa 的生长速率为0.40 d⁻¹，每天产生226.3 mg·L⁻¹的生物量，其中含有脂质（487.26 mg gdw⁻¹）、叶黄素（7.07 mg gdw⁻¹）和FAMEs（232.9 mg gdw⁻¹）；48.4%的FAME为PUFAs。PAR +紫外线B（UVB，0.16 W m⁻²）的光照对细胞有毒。这些结果表明，卵形 coccomyxa 生物量适合作为功能性食品的补充剂和/或高附加值产品的来源。
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