一种用于 sp. FSP3 的两段式培养策略，以实现 CO2 固定和在光照及盐胁迫下同时生产叶黄素。
A Two-Stage Culture Strategy for sp. FSP3 for CO Fixation and the Simultaneous Production of Lutein under Light and Salt Stress.
机构信息
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China.
出版信息
Molecules. 2022 Nov 3;27(21):7497. doi: 10.3390/molecules27217497.
In this study, sp. FSP3 was cultured using a two-stage culture strategy for CO fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO introduced to promote the rapid growth and CO fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L and a light intensity of 160 μmol m s were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of sp. FSP3 reached 0.58 g L d and 1.09 g L d, with a lutein content and yield as high as 6.45 mg g and 2.30 mg L d, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO fixation processes.
在这项研究中，采用两阶段培养策略来培养 sp. FSP3，以进行 CO 固定和叶黄素生产。在第一阶段，向培养基中添加碳酸丙烯酯，并引入 5%的 CO，以促进微藻的快速生长和 CO 固定。在第二阶段的培养中，使用 156 mmol L 的 NaCl 浓度和 160 μmol m s 的光强度来刺激微藻细胞中叶黄素的积累。通过使用这种培养方法，同时实现了高叶黄素生产和 CO 固定。sp. FSP3 的生物量生产力和碳固定率分别达到 0.58 g L d 和 1.09 g L d，叶黄素含量和产量分别高达 6.45 mg g 和 2.30 mg L d。研究结果表明，将微藻叶黄素生产与 CO 固定过程相结合具有商业可行性。
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