College of Veterinary Medicine, Hunan Collaborative Innovation Center of Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, China.
College of Veterinary Medicine, Hunan Collaborative Innovation Center of Safety Production of Livestock and Poultry, Hunan Agricultural University, Changsha, China.
Ticks Tick Borne Dis. 2018 Mar;9(3):490-495. doi: 10.1016/j.ttbdis.2018.01.008. Epub 2018 Jan 20.
Scant information is available regarding the proteins involved in blood meal processing in ticks. Here, we aimed to highlight the midgut proteins involved in preventing blood meal coagulation, and in facilitating intracellular digestion in the tick Haemaphysalis flava. Proteins were extracted from the midgut contents of fully engorged and partially engorged ticks. We used liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify 131 unique peptides, and 102 proteins. Of these, 15 proteins, each with at least two unique peptides, were recognized with high confidence. We also retrieved 18 unigenes from our previous published transcriptomic libraries of the midguts and salivary glands of H. flava, and inferred the primary structures of nine proteins and fragments of five proteins. There were 23 and 21 unique proteins in the midgut contents of fully engorged and partially engorged ticks, respectively. We detected 58 shared proteins in the midgut contents of both fully engorged and partially engorged ticks. Of these, seven were significantly differentially expressed between fully engorged and partially engorged ticks: actin, calmodulin, elongation factor-1α, hsp90, multifunctional chaperone, tubulin α, and tubulin β. Our results demonstrated that the proteome of the midgut contents, combined with the transcriptome of the midgut, was a viable method for the reinforcement of protein identification. This method will facilitate further study of blood meal processing by ticks, as well as the identification of clues for tick infestation control. The existence of numerous proteins detected in the midgut contents also highlight the complexity of blood digestion in ticks; this area is in need of further investigation.
关于蜱中参与血餐处理的蛋白质，相关信息很少。在这里，我们旨在强调参与防止血餐凝结和促进蜱 Haemaphysalis flava 细胞内消化的中肠蛋白。从中肠内容物中提取了完全饱血和部分饱血的蜱的蛋白质。我们使用液相色谱串联质谱（LC-MS/MS）分析鉴定了 131 个独特肽和 102 个蛋白质。其中，15 个蛋白质，每个蛋白质都有至少两个独特的肽，被高度置信地识别。我们还从我们之前发表的 Haemaphysalis flava 中肠和唾液腺转录组文库中检索到 18 个 unigenes，并推断了 9 个蛋白质的一级结构和 5 个蛋白质片段。完全饱血和部分饱血的蜱的中肠内容物分别有 23 个和 21 个独特蛋白质。我们在完全饱血和部分饱血的蜱的中肠内容物中检测到 58 个共同蛋白质。其中，7 个在完全饱血和部分饱血的蜱之间差异表达显著：肌动蛋白、钙调蛋白、延伸因子-1α、热休克蛋白 90、多功能伴侣蛋白、微管蛋白α和微管蛋白β。我们的结果表明，中肠内容物的蛋白质组与中肠的转录组相结合，是增强蛋白质鉴定的可行方法。这种方法将有助于进一步研究蜱的血餐处理以及寻找控制蜱侵害的线索。在中肠内容物中检测到的大量蛋白质的存在也突出了蜱中血液消化的复杂性；这一领域需要进一步研究。
