Grönhagen-Riska C, von Willebrand E, Tikkanen I, Miettinen A, Törnroth T
Scand J Urol Nephrol Suppl. 1985;90:35-40.
Fine-needle aspiration biopsy (FNAB) has been successfully used for the monitoring of the in situ inflammatory response of rejection. We have evaluated FNAB as a technique for the quantification and cytological classification of infiltrating leukocytes during the induction of and manifest Heymann nephritis (HN). FNAB results were compared and supplemented with results of conventional histopathological and immunofluorescence (IFL) studies and with immunohistochemical studies to classify infiltrating T-lymphocyte subsets with immunoperoxidase (IP) technique. Rats (8/group) were killed three weeks after immunisation, and two and ten weeks after a booster. FNAB proved more sensitive than histopathological examination for the evaluation of interstitial infiltration in HN. Plasmablasts dominated the inflammatory picture three weeks after immunisation, when no glomerular or interstitial changes were observed histopathologically. The intensity and number of plasmablast reactions reached their peak two weeks after the booster, although disease was still not apparent. When membranous glomerulonephritis (MGN) was established, FNAB indicated plasmablasts in 4/7 rats, the reaction was strong in two. These rats had the most severe histopathological changes, too. FNAB indicated modest lymphocyte infiltration three weeks after immunisation. Immunohistochemical subtyping of T-lymphocytes showed initial T-helper infiltration, which gradually decreased after the booster, and had disappeared when MGN was manifest. At this stage T-suppressor infiltration was observed in the same two rats which had strong plasmablast reactions and severe HN with interstitial changes, as well. These observations suggest a role for cell-bound immunity in the induction of HN. Plasmablast reaction may initially be supported by the infiltrating T-helper lymphocytes, and it may imply local antibody production. Severe manifest disease was associated with both plasmablast and suppressor cell infiltration.
细针穿刺活检（FNAB）已成功用于监测排斥反应的原位炎症反应。我们评估了FNAB作为一种在海曼肾炎（HN）诱导期和明显期对浸润白细胞进行定量和细胞分类的技术。将FNAB结果与传统组织病理学和免疫荧光（IFL）研究结果以及免疫组织化学研究结果进行比较，并通过免疫过氧化物酶（IP）技术对浸润的T淋巴细胞亚群进行分类。每组8只大鼠在免疫后3周、加强免疫后2周和10周处死。在评估HN的间质浸润方面，FNAB比组织病理学检查更敏感。免疫后3周，成浆细胞在炎症表现中占主导地位，此时组织病理学上未观察到肾小球或间质变化。尽管疾病仍不明显，但成浆细胞反应的强度和数量在加强免疫后2周达到峰值。当膜性肾小球肾炎（MGN）形成时，FNAB显示4/7的大鼠有成浆细胞，其中2只反应强烈。这些大鼠也有最严重的组织病理学变化。FNAB显示免疫后3周有适度的淋巴细胞浸润。T淋巴细胞的免疫组织化学亚型分析显示最初有T辅助细胞浸润，加强免疫后逐渐减少，在MGN明显时消失。在这个阶段，在同样两只有成浆细胞强烈反应和严重HN伴间质变化的大鼠中也观察到了T抑制细胞浸润。这些观察结果表明细胞结合免疫在HN诱导中起作用。成浆细胞反应最初可能由浸润的T辅助淋巴细胞支持，这可能意味着局部抗体产生。严重的明显疾病与成浆细胞和抑制细胞浸润都有关。
