在图案化双功能表面上的正交超分子蛋白质组装。
Orthogonal supramolecular protein assembly on patterned bifunctional surfaces.
机构信息
Bioinspired Molecular Engineering Laboratory, MIRA Biomedical Technology and Technical Medicine Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Nanobiophysics Group, MESA+ Institute for Nanotechnology and MIRA Biomedical Technology and Technical Medicine Institute, University of Twente, 7500 AE Enschede, The Netherlands and Free University of Amsterdam, De Boelelaan 1105, 1081 HV, Amsterdam, The Netherlands.
出版信息
Chem Commun (Camb). 2018 Feb 14;54(13):1615-1618. doi: 10.1039/c7cc09808a. Epub 2018 Jan 26.
We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.
我们报告了在具有双功能 β-CD-Ni(ii)NTA 图案的表面上成功且选择性的双重蛋白质组装，使用带有六组氨酸标签的红色荧光蛋白变体和与包含三个金刚烷基的肽缀合的青色荧光蛋白变体。我们表明，只有当优化了相反的超分子相互作用并充分抑制了非特异性相互作用时，才能组装双重蛋白质图案。
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