Harmansa Stefan, Affolter Markus
Growth and Development, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Growth and Development, Biozentrum, University of Basel, 4056 Basel, Switzerland
Development. 2018 Jan 26;145(2):dev148874. doi: 10.1242/dev.148874.
Developmental biology research would benefit greatly from tools that enable protein function to be regulated, both systematically and in a precise spatial and temporal manner, In recent years, functionalized protein binders have emerged as versatile tools that can be used to target and manipulate proteins. Such protein binders can be based on various scaffolds, such as nanobodies, designed ankyrin repeat proteins (DARPins) and monobodies, and can be used to block or perturb protein function in living cells. In this Primer, we provide an overview of the protein binders that are currently available and highlight recent progress made in applying protein binder-based tools in developmental and synthetic biology.
发育生物学研究将从能够系统地、精确地在空间和时间上调节蛋白质功能的工具中受益匪浅。近年来，功能化蛋白质结合物已成为可用于靶向和操纵蛋白质的多功能工具。此类蛋白质结合物可基于各种支架，如纳米抗体、设计锚蛋白重复蛋白（DARPins）和单域抗体，可用于阻断或干扰活细胞中的蛋白质功能。在本入门指南中，我们概述了目前可用的蛋白质结合物，并重点介绍了在发育生物学和合成生物学中应用基于蛋白质结合物的工具所取得的最新进展。
