Panagia V, Okumura K, Makino N, Dhalla N S
Biochim Biophys Acta. 1986 Apr 14;856(2):383-7. doi: 10.1016/0005-2736(86)90049-0.
Incubation of purified cardiac sarcolemmal vesicles (SL) in the presence of S-adenosyl-L-methionine, a methyl donor for the enzymatic N-methylation of phosphatidylethanolamine (PE), increased the Ca2+-stimulated ATPase and ATP-dependent Ca2+ accumulation activities. Quantitative analysis of the methylated phospholipids revealed that maximal increase of Ca2+-pump activities was associated with predominant synthesis and intramembranal accumulation of phosphatidyl-N,N-dimethylethanolamine. The stimulation of SL Ca2+-pump activities was prevented by inhibitors of PE N-methylation such as S-adenosyl-L-homocysteine and methyl acetimidate hydrochloride. The results suggest a possible role of PE N-methylation in the regulation of Ca2+-transport across the heart SL membrane.
在作为磷脂酰乙醇胺（PE）酶促N - 甲基化甲基供体的S - 腺苷 - L - 甲硫氨酸存在下，对纯化的心肌肌膜囊泡（SL）进行孵育，可增加Ca2 + 刺激的ATP酶活性以及ATP依赖性Ca2 + 积累活性。对甲基化磷脂的定量分析表明，Ca2 + 泵活性的最大增加与磷脂酰 - N，N - 二甲基乙醇胺的主要合成及膜内积累有关。PE N - 甲基化抑制剂如S - 腺苷 - L - 高半胱氨酸和盐酸甲基乙酰亚胺可阻止SL Ca2 + 泵活性的刺激。结果表明PE N - 甲基化在调节Ca2 + 跨心肌SL膜转运中可能发挥作用。
