维生素D与COX2抑制剂联合治疗对乳腺癌细胞系的影响
Effects of Combined Treatment with Vitamin D and COX2 Inhibitors on Breast Cancer Cell Lines.
作者信息
Friedrich Michael, Reichert Kathrin, Woeste Alena, Polack Stephan, Fischer Dorothea, Hoellen Friederike, Rody Achim, Köster Frank, Thill Marc
机构信息
Department of Gynecology and Obstetrics, Helios Hospital Krefeld, Krefeld, Germany
Department of Gynecology and Obstetrics, University of Lübeck, Lübeck, Germany.
出版信息
Anticancer Res. 2018 Feb;38(2):1201-1207. doi: 10.21873/anticanres.12340.
BACKGROUND
Vitamin D is known for its anticancer potential. Prostaglandin E (PGE) is a proliferative and inflammation-activating agent. The production of PGE is dependent on the activity of cyclooxygenase-2 (COX2). A link between vitamin D and PGE metabolism was shown recently.
MATERIALS AND METHODS
In MDA-MB-231 and MCF-7 breast cancer cell lines, we investigated the influence of calcitriol and the COX2 inhibitor celecoxib on cell growth via the MTT test, as well as on the protein and mRNA expression of COX2 using western blot and quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
The proliferation of MCF-7 and MDA-MB-231 was inhibited by both calcitriol and the COX2 inhibitor celecoxib and even more strongly by their combination. Moreover, calcitriol inhibited COX2 protein expression in MDA-MB-231 cells, as well as COX2 mRNA expression in both cell lines.
CONCLUSION
The combination of calcitriol and celecoxib demonstrated a synergistic growth-inhibitory effect in breast cancer cell lines.
背景
维生素D以其抗癌潜力而闻名。前列腺素E（PGE）是一种增殖和炎症激活剂。PGE的产生依赖于环氧合酶-2（COX2）的活性。最近发现维生素D与PGE代谢之间存在联系。
材料与方法
在MDA-MB-231和MCF-7乳腺癌细胞系中，我们通过MTT试验研究了骨化三醇和COX2抑制剂塞来昔布对细胞生长的影响，并使用蛋白质印迹法和定量实时聚合酶链反应（qRT-PCR）研究了它们对COX2蛋白和mRNA表达的影响。
结果
骨化三醇和COX2抑制剂塞来昔布均抑制MCF-7和MDA-MB-231的增殖，二者联合使用时抑制作用更强。此外，骨化三醇抑制MDA-MB-231细胞中COX2蛋白表达以及两种细胞系中COX2 mRNA表达。
结论
骨化三醇和塞来昔布联合使用在乳腺癌细胞系中显示出协同生长抑制作用。
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