病毒性心肌炎中的微小RNA信号传导：新的独特病理特征
MiRNA Signaling in Viral Myocarditis Novel and Unique Pathological Features.
作者信息
Wang Yu, Wei Cheng-Xi, Shao Li-Qun, Zhao Ming
机构信息
Inner Mongolia University for the Nationalities.
Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for Cardio-Cerebral Vascular System, Tongliao, Inner Mongolia, P.R. China.
出版信息
Acta Cardiol Sin. 2018 Jan;34(1):77-86. doi: 10.6515/ACS.201801_34(1).20170901A.
BACKGROUND
Micro-RNAs (miRNAs) are small non-coding RNAs that modulate many target genes. Viral myocarditis is common cardiomyopathy, however, there is an absence of effective therapeutic strategies for viral myocarditis (VMC). The purpose of this research was to characterize changes in miRNAs expression in VMC mice.
METHODS
Atrial myocytes were infected coxsackievirus B3 and miRNAs microarray was performed. miRNAs target predicted and the bioinformatics analysis was carried out by gene ontology (GO) and KEGG pathway analysis. To validate the results, Difference miRNAs were identified in heart of mice by real-time polymerase chain reaction (PCR).
RESULTS
We identified 94 miRNAs that were differentially expressed (27 were up-regulated and 67 were down-regulated by at least 2.0-fold). Real time PCR analysis has confirmed that the expression levels of 7 miRNAs up-regulated, 18 miRNAs down-regulated. They were mainly involved in protein binding, small GTPase mediated signal transduction, protein phosphorylation by GO. Pathway analysis showed that a significant enrichment in several pathways related to cAMP signaling pathway, AMPK signaling pathway, RAS signaling pathway, Rap1 signaling pathway, ErbB signaling pathway, Oxytocin signaling pathway.
CONCLUSIONS
Our results provide a better understanding of the mechanisms of viral myocarditis pathophysiology.
背景
微小RNA（miRNA）是一类可调控众多靶基因的小型非编码RNA。病毒性心肌炎是常见的心肌病，但目前缺乏针对病毒性心肌炎（VMC）的有效治疗策略。本研究旨在明确VMC小鼠中miRNA表达的变化情况。
方法
用柯萨奇病毒B3感染心房肌细胞并进行miRNA微阵列分析。通过基因本体论（GO）和KEGG通路分析对miRNA靶标进行预测并开展生物信息学分析。为验证结果，采用实时聚合酶链反应（PCR）在小鼠心脏中鉴定差异miRNA。
结果
我们鉴定出94个差异表达的miRNA（27个上调，67个下调至少2.0倍）。实时PCR分析证实7个miRNA上调、18个miRNA下调。GO分析显示它们主要参与蛋白质结合、小GTP酶介导的信号转导、蛋白质磷酸化。通路分析表明，与cAMP信号通路、AMPK信号通路、RAS信号通路、Rap1信号通路、ErbB信号通路、催产素信号通路相关的多个通路有显著富集。
结论
我们的结果有助于更好地理解病毒性心肌炎病理生理学机制。
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