Matsui Y, Staunton D E, Shapiro H M, Yunis E J
Hum Immunol. 1986 Mar;15(3):285-301. doi: 10.1016/0198-8859(86)90004-2.
Although it is well known that the expression of major histocompatibility complex (MHC) antigens on the surface of lymphoblastoid cell lines are cell cycle dependent, the way in which the MHC antigen expression on activated T cells varies with cell cycle phase has not previously been described. Using 11 lymphoblastoid cell lines from malignant and nonmalignant tissues (B cells, T cells, and myeloid cells) and five activated T cell lines (two cell lines activated by phytohemagglutinin and three alloreactive T cell clones), MHC antigen expression was quantitatively studied by dual-beam flow cytometry. Correlated measurements of surface antigen quantity (immunofluorescence), DNA content (Hoechst 33342), and cell size (light scatter), uninfluenced by induction synchrony and cell fixation, were performed. The data indicate that cell surface antigen quantity and cell surface area demonstrate specific values at each phase of the cell cycle when the cells are in logarithmic growth. Examining cells in logarithmic growth, it was confirmed, for all lymphoblastoid cell lines, that the quantity of MHC antigens on G2 (S + G2 + M) cells was greater than that on G1 cells. In addition, it was found, by analyzing antigen quantity and surface area, that class I antigen density in the G2 phase is 17% less than that in the G1 phase in leukemic T cell lines, and that both class I and class II antigen densities in the G2 phase were 21% less than that in the G1 phase in lymphoblastoid B cell lines. In activated T cells, class I antigen density in the G2 phase was 11% less than that in the G1 phase, while class II antigen density in the G2 phase was 12% greater than that in the G1 phase. We describe four important observations in this report. In both G1 and G2 phases, activated T cells express: quantitatively fewer class I antigens than lymphoblastoid B cell lines; similar quantity of class I antigens as that of leukemic T cell lines; and similar quantity of class II antigens as that of lymphoblastoid B cell lines. Also, class II antigens are expressed in greater density in the G2 phase than in the G1 phase in activated T cells. In contrast, lymphoblastoid B cell lines express greater density of class II antigens in the G1 phase than in the G2 phase of the cell cycle. These findings differ from previous reports, suggesting that G1 phase cells may have a more significant role than G2 phase cells as target cells for MHC restricted cytotoxic cells.
虽然众所周知，淋巴母细胞系表面主要组织相容性复合体（MHC）抗原的表达是细胞周期依赖性的，但活化T细胞上MHC抗原表达随细胞周期阶段变化的方式此前尚未见报道。利用来自恶性和非恶性组织的11个淋巴母细胞系（B细胞、T细胞和髓细胞）以及5个活化T细胞系（2个由植物血凝素激活的细胞系和3个同种异体反应性T细胞克隆），通过双光束流式细胞术对MHC抗原表达进行了定量研究。进行了表面抗原量（免疫荧光）、DNA含量（Hoechst 33342）和细胞大小（光散射）的相关测量，不受诱导同步化和细胞固定的影响。数据表明，当细胞处于对数生长期时，细胞表面抗原量和细胞表面积在细胞周期的每个阶段都显示出特定的值。检查处于对数生长期的细胞，对于所有淋巴母细胞系，均证实G2期（S + G2 + M期）细胞上的MHC抗原量大于G1期细胞。此外，通过分析抗原量和表面积发现，白血病T细胞系中G2期的I类抗原密度比G1期低17%，淋巴母细胞B细胞系中G2期的I类和II类抗原密度均比G1期低21%。在活化T细胞中，G2期的I类抗原密度比G1期低11%，而G2期的II类抗原密度比G1期高12%。我们在本报告中描述了四个重要观察结果。在G1期和G2期，活化T细胞均表达：I类抗原的数量比淋巴母细胞B细胞系少；I类抗原的数量与白血病T细胞系相似；II类抗原的数量与淋巴母细胞B细胞系相似。此外，活化T细胞中G2期II类抗原的表达密度高于G1期。相比之下，淋巴母细胞B细胞系在细胞周期的G1期II类抗原的表达密度高于G2期。这些发现与先前的报道不同，表明G1期细胞作为MHC限制性细胞毒性细胞的靶细胞可能比G2期细胞发挥更重要的作用。
