Leung D Y, Young M C, Geha R S
J Immunol. 1986 Apr 15;136(8):2851-5.
During the course of generating tetanus toxoid (TT)-specific T cell clones frm an HLA-DR2,7 donor, four clones were obtained which proliferated in the presence of autologous monocytes alone without the addition of TT antigen. This proliferation was specifically inhibited by anti-HLA-DR framework mouse monoclonal antibody, and appeared to be HLA-DR-restricted. Two of the clones proliferated in response to HLA-DR2-bearing monocytes, and the other two clones proliferated in response to HLA-DR7-bearing monocytes. The capacity of these four autoreactive human T cell helper clones to induce IgE synthesis in B cells was studied. All four clones stimulated autologous peripheral blood B cells to synthesize IgE and IgG antibody. Induction of IgE synthesis in B cells by the autoreactive T cell clones followed the same pattern of HLA-DR restriction which governed the proliferative response of these clones. These results suggest that the interaction of autoreactive helper T cells with B cell HLA-DR antigens may be important in the activation of IgE immune responses in humans.
在从一名HLA - DR2、7供体生成破伤风类毒素（TT）特异性T细胞克隆的过程中，获得了四个克隆，它们在仅存在自体单核细胞而未添加TT抗原的情况下就能增殖。这种增殖被抗HLA - DR构架小鼠单克隆抗体特异性抑制，并且似乎受HLA - DR限制。其中两个克隆对携带HLA - DR2的单核细胞有增殖反应，另外两个克隆对携带HLA - DR7的单核细胞有增殖反应。研究了这四个自身反应性人类T细胞辅助克隆在B细胞中诱导IgE合成的能力。所有四个克隆都刺激自体外周血B细胞合成IgE和IgG抗体。自身反应性T细胞克隆在B细胞中诱导IgE合成遵循与这些克隆增殖反应相同的HLA - DR限制模式。这些结果表明，自身反应性辅助性T细胞与B细胞HLA - DR抗原的相互作用可能在人类IgE免疫反应的激活中起重要作用。
