Li Shilin, Pasquin Sarah, Eid Hoda M, Gauchat Jean-François, Saleem Ammar, Haddad Pierre S
Department of Pharmacology and Physiology, Université de Montréal, P.O. Box 6128, Downtown Postal Station, Montreal, (Quebec), H3C 3J7, Canada.
Natural Health Products and Metabolic Diseases Laboratory, CIHR Team in Aboriginal Antidiabetic Medicines and Montreal Diabetes Research Center, CRCHUM, Montreal, Canada.
BMC Complement Altern Med. 2018 Jan 30;18(1):37. doi: 10.1186/s12906-018-2104-1.
Our team has identified 17 Boreal forest species from the traditional pharmacopeia of the Eastern James Bay Cree that presented promising in vitro and in vivo biological activities in the context of type 2 diabetes (T2D). We now screened the 17 plants extracts for potential anti-apoptotic activity in cultured kidney cells and investigated the underlying mechanisms.
MDCK (Madin-Darnby Canine Kidney) cell damage was induced by hypertonic medium (700 mOsm/L) in the presence or absence of maximal nontoxic concentrations of each of the 17 plant extracts. After 18 h' treatment, cells were stained with Annexin V (AnnV) and Propidium iodide (PI) and subjected to flow cytometry to assess the cytoprotective (AnnV/PI) and anti-apoptotic (AnnV/PI) potential of the 17 plant extracts. We then selected a representative subset of species (most cytoprotective, moderately so or neutral) to measure the activity of caspases 3, 8 and 9.
Gaultheria hispidula and Abies balsamea are amongst the most powerful cytoprotective and anti-apoptotic plants and appear to exert their modulatory effect primarily by inhibiting caspase 9 in the mitochondrial apoptotic signaling pathway.
We conclude that several Cree antidiabetic plants exert anti-apoptotic activity that may be relevant in the context of diabetic nephropathy (DN) that affects a significant proportion of Cree diabetics.
我们的团队从东詹姆斯湾克里人的传统药典中鉴定出17种北方森林物种，这些物种在2型糖尿病（T2D）背景下展现出了有前景的体外和体内生物学活性。我们现在对这17种植物提取物进行培养的肾细胞中潜在抗凋亡活性的筛选，并研究其潜在机制。
在存在或不存在17种植物提取物各自的最大无毒浓度的情况下，用高渗培养基（700 mOsm/L）诱导MDCK（麦迪逊-达比犬肾）细胞损伤。处理18小时后，用膜联蛋白V（AnnV）和碘化丙啶（PI）对细胞进行染色，并进行流式细胞术以评估17种植物提取物的细胞保护（AnnV/PI）和抗凋亡（AnnV/PI）潜力。然后我们选择了一个具有代表性的物种子集（细胞保护作用最强、中等或无作用）来测量半胱天冬酶3、8和9的活性。
毛高梁泡和香脂冷杉是细胞保护和抗凋亡作用最强的植物之一，并且似乎主要通过抑制线粒体凋亡信号通路中的半胱天冬酶9来发挥其调节作用。
我们得出结论，几种克里族抗糖尿病植物具有抗凋亡活性，这可能与影响相当一部分克里族糖尿病患者的糖尿病肾病（DN）有关。
