欧米加-3 脂肪酸（鱼油）、二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）和奥沙利铂对食管腺癌细胞系生长、细胞因子和细胞信号生物标志物表达的影响。

Effects of Omegaven®, EPA, DHA and oxaliplatin on oesophageal adenocarcinoma cell lines growth, cytokine and cell signal biomarkers expression.

机构信息

Department of Surgery, University Hospitals of Leicester NHS Trust, Leicester Royal Infirmary, Leicester, LE1 5WW, UK.

Department of Cancer Studies, University of Leicester, Leicester, LE2 7LX, UK.

出版信息

Lipids Health Dis. 2018 Jan 30;17(1):19. doi: 10.1186/s12944-018-0664-1.

BACKGROUND

There is limited evidence assessing the effects of omega-3 polyunsaturated fatty acids (PUFAs) on oesophageal adenocarcinoma, both in vitro and in vivo. We evaluated the effects of the omega-3 PUFA and oxaliplatin on OE33 and OE19 cells.

METHOD

The two oesophageal cells were treated with Omegaven® (fish oil emulsion), EPA, DHA and oxaliplatin and incubated for up to 144 h.

RESULTS

The following inhibitory effects were observed on OE33 cells: EPA reduced cell growth by 39% (p = 0.001), DHA by 59% (p < 0.000) and Oxaliplatin by 77% (p < 0.000). For OE19 cells, the EPA reduced growth by 1% (p = 0.992), DHA by 26% (p = 0.019) and oxaliplatin by 76% (p < 0.000). For both cells, Omegaven® resulted in reduced cell growth at intermediate concentrations (20-40 μM) and increased cell growth at low (10 μM) and high (50 μM) concentrations. DHA, Omegaven® and oxaliplatin were associated with significant downregulation of VEGF and p53 protein, and upregulation of p21 protein. DHA, Omegaven® and Oxaliplatin also led to significant downregulation of the total ERK1/2 and Akt proteins.

CONCLUSION

DHA, Omegaven® and oxaliplatin were associated with downregulation of p53 and VEGF in both cells. Of the PUFAs studied, DHA alone or in combination (Omegaven®) had greater in vitro anti-cancer effects than EPA alone.

背景

目前仅有有限的研究证据评估了ω-3 多不饱和脂肪酸（PUFA）在体外和体内对食管腺癌的影响。我们评估了ω-3PUFA 和奥沙利铂对 OE33 和 OE19 细胞的作用。

方法

将两种食管细胞用 Omegaven®（鱼油乳剂）、EPA、DHA 和奥沙利铂处理并孵育长达 144 小时。

结果

OE33 细胞观察到以下抑制作用：EPA 使细胞生长减少 39%（p = 0.001），DHA 使细胞生长减少 59%（p < 0.000），奥沙利铂使细胞生长减少 77%（p < 0.000）。对于 OE19 细胞，EPA 使细胞生长减少 1%（p = 0.992），DHA 使细胞生长减少 26%（p = 0.019），奥沙利铂使细胞生长减少 76%（p < 0.000）。对于两种细胞，Omegaven®在中等浓度（20-40 μM）时导致细胞生长减少，在低浓度（10 μM）和高浓度（50 μM）时导致细胞生长增加。DHA、Omegaven®和奥沙利铂与 VEGF 和 p53 蛋白的显著下调以及 p21 蛋白的显著上调相关。DHA、Omegaven®和奥沙利铂还导致总 ERK1/2 和 Akt 蛋白的显著下调。