Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Analyst. 2018 Feb 12;143(4):833-836. doi: 10.1039/c7an02044a.
We use 2D UV-MS cold-ion spectroscopy for the identification of l-Asp, d-Asp, l-isoAsp and d-isoAsp residues in a fragment peptide derived from the hormone protein amylin. Relative solution concentrations of all four isoforms in an equimolar quaternary mixture have been determined within 4% error. This method demonstrates that for binary mixtures of the peptides an accuracy of 2.5% can be reached in few-second measurements.
我们使用 2D UV-MS 冷离子光谱法鉴定从激素蛋白胰淀素衍生的片段肽中的 l-Asp、d-Asp、l-isoAsp 和 d-isoAsp 残基。在等摩尔四元混合物中，已确定所有四种异构体的相对溶液浓度的误差在 4%以内。该方法表明，对于肽的二元混合物，在几秒钟的测量中可以达到 2.5%的精度。
