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[骚扰锥蝽发育周期的生物化学。VII. 用己烷提取的表皮表面的脂质组成]

[Biochemistry of the developmental cycle of Triatoma infestans. VII. Lipid composition of the cuticle surface extracted with hexane].

作者信息

Juárez P, Brenner R R, Labayén I L, Gros E G

出版信息

Acta Physiol Pharmacol Latinoam. 1985;35(2):223-36.

PMID:2938407
Abstract

The lipid composition of Triatoma infestans (vinchuca) epicuticle was studied. The insect lipids were extracted with hexane and fractionated by chromatography. Long chain fatty alcohols, hydrocarbons, waxes, triacylglycerols, diacylglycerols, free fatty acids, cholesterol, cholesterol esters and monoacylglycerols were detected. The composition of fatty acids, hydrocarbons and alcohols was studied by gas-liquid chromatography and mass spectrography. Linear and branched hydrocarbons were separated. Both types had an uneven number of up to 41 carbons. The predominant straight-chain hydrocarbons were in the range of 27 to 33 carbons. The branched hydrocarbons identified were 2-methyl, 3-methyl and internally branched monomethyl-alkanes and dimethyl-alkanes. The fatty alcohols identified had a straight chain of 16 to 34 carbons with predominance of 28 to 34 specimens. In the different fractions of esterified fatty acids and in the free acids two groups were detected: one formed by acids of 16 to 18 carbons saturated and non-saturated, and another by very long chain saturated acids of 24 to 32 carbons. The presence of alcohols, hydrocarbons and waxes, constituted mainly by long and saturated chains, conveys to the cuticle high resistance to physical and chemical aggressors of the environment.

摘要

对骚扰锥蝽(vinchuca)表皮的脂质成分进行了研究。用己烷提取昆虫脂质,并用色谱法进行分离。检测到长链脂肪醇、碳氢化合物、蜡、三酰甘油、二酰甘油、游离脂肪酸、胆固醇、胆固醇酯和单酰甘油。通过气液色谱法和质谱法研究了脂肪酸、碳氢化合物和醇的组成。分离出了直链和支链碳氢化合物。这两种类型的碳氢化合物碳原子数均为奇数,最多达41个。主要的直链碳氢化合物碳原子数在27至33个之间。鉴定出的支链碳氢化合物为2-甲基、3-甲基和内部支链的单甲基烷烃和二甲基烷烃。鉴定出的脂肪醇具有16至34个碳原子的直链,其中以28至34个碳原子的为主。在酯化脂肪酸的不同馏分和游离酸中检测到两组:一组由16至18个碳原子的饱和与不饱和酸组成,另一组由24至32个碳原子的非常长链的饱和酸组成。醇、碳氢化合物和蜡的存在,主要由长链和饱和链构成,使表皮对环境的物理和化学侵害具有高度抗性。

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