Pujol C A
Acta Physiol Pharmacol Latinoam. 1985;35(2):251-8.
Two related species of the genus Ceratophrys (C. ornata and C. cranwelli) were studied by immunological techniques in order to find non-morphological characters which would allow to classify them more precisely. Bidimensional immunodiffusion of serum proteins showed extensive crossreactivity between both species, thus revealing their close relatíonship. However, by immunoelectrophoretic techniques, differences between their serum fractions were detected. The antisera raised in rabbits against the serum proteins showed different potency in reacting with the sera of each species. Sharper precipitation bands were obtained with the homologous serum. Two lipoproteic fractions, in the serum of both species, were also characterized.
为了找到能够更精确地对角蛙属（饰纹角花蟾和克兰威尔角花蟾）的两个近缘物种进行分类的非形态学特征，我们采用免疫技术对它们进行了研究。血清蛋白的双向免疫扩散显示这两个物种之间存在广泛的交叉反应，从而揭示了它们的密切关系。然而，通过免疫电泳技术，我们检测到了它们血清组分之间的差异。用兔制备的针对血清蛋白的抗血清在与每个物种的血清反应时表现出不同的效价。与同源血清反应时获得了更清晰的沉淀带。我们还对这两个物种血清中的两种脂蛋白组分进行了表征。
