Fichera L E, Brenner R R
Acta Physiol Pharmacol Latinoam. 1985;35(3):319-25.
The three lipoproteins of high (HDL) and very high density (VHDL-I and VHDL-II) were separated from the hemolymph of adult males of T. infestans. They were delipidated and the corresponding apolipoproteins examined by sodium dodecyl sulphate (SDS) gel electrophoresis in polyacrylamide in the presence of 2-mercaptoethanol. They were also iso-electro focused in a pH gradient of 5 to 8. All three groups of apolipoproteins were separated in several polypeptide chains, but all of them had in common a glycoproteic unit of PM 86 000. The VHDL-II presented the simplest composition with predominance of the 86 000 band. Apo-HDL was the most complicated and showed intense bands of PM as high as 210 000 and as low as 17 000 together with 44 000 and 86 000 and less intense bands. The apo-VHDL-I was separated in polypeptides chains in the range of 86 000 to 17 000. The different apoproteins are apparently formed by the association at least in part of common polypeptides.
从致倦库蚊成年雄虫的血淋巴中分离出了三种脂蛋白，即高密度脂蛋白（HDL）和两种极高密度脂蛋白（VHDL-I和VHDL-II）。对它们进行脱脂处理，并在2-巯基乙醇存在的情况下，通过十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳对相应的载脂蛋白进行检测。还在pH值为5至8的梯度中进行等电聚焦。所有三组载脂蛋白都被分离成几条多肽链，但它们都有一个分子量为86000的糖蛋白单元。VHDL-II的组成最简单，以86000条带为主。载脂蛋白-HDL最为复杂，显示出分子量高达210000和低至17000的强条带，以及44000和86000的条带，还有较弱的条带。载脂蛋白-VHDL-I被分离成分子量在86000至17000范围内的多肽链。不同的载脂蛋白显然至少部分是由共同多肽的缔合形成的。
