用于生产β-己基葡萄糖苷的BC9β-葡萄糖苷酶。

BC9 β-Glucosidase for the Production of β--Hexyl Glucoside.

作者信息

Sujittra Sujittra, Vichitphan Kanit, Han Jaehong, Vichitphan Sukanda, Swangkaew Jutaporn

机构信息

Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.

Department of Biotechnology and Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen 40002, Thailand.

出版信息

J Microbiol Biotechnol. 2018 Apr 28;28(4):579-587. doi: 10.4014/jmb.1712.12037.

DOI:10.4014/jmb.1712.12037

PMID:29385667

Abstract

For biotechnological production of high-valued β--hexyl glucoside, the catalytic properties of BC9 β-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of β--hexyl glucoside was optimized. The constitutive BC9 β-glucosidase exhibited maximum specific activity at pH 6.0 and 40ºC, and the activity of BC9 β-glucosidase was not significantly inhibited by various metal ions. BC9 β-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 β-glucosidase exhibited enhanced production of β--hexyl glucoside in the presence of DMSO, and 62% of β--hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.

摘要

为了通过生物技术生产高价值的β-己基葡萄糖苷，研究了从周质组分中纯化得到的BC9β-葡萄糖苷酶的催化特性，并对生产β-己基葡萄糖苷的转糖基化活性进行了优化。组成型BC9β-葡萄糖苷酶在pH 6.0和40℃时表现出最大比活性，并且BC9β-葡萄糖苷酶的活性不受各种金属离子的显著抑制。BC9β-葡萄糖苷酶没有显示出明显的纤维二糖水解活性，但在蔗糖和中链醇存在下活性反而增强。BC9β-葡萄糖苷酶在二甲基亚砜（DMSO）存在下β-己基葡萄糖苷的产量增加，在5% 1-己醇和15% DMSO存在下4小时内记录到62%的β-己基葡萄糖苷转化率。