Kumar Ashok, Patel Sanjay K S, Mardan Bharat, Pagolu Raviteja, Lestari Rowina, Jeong Seong-Hoon, Kim Taedoo, Haw Jung Rim, Kim Sang-Yong, Kim In-Won, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Institute of SK-KU Biomaterials, Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2018 Apr 28;28(4):638-644. doi: 10.4014/jmb.1710.10037.
In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at 4°C for 72 h, using 0.25 mg/ml protein, and efficient immobilization of xylanase was observed, with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. Immobilized xylanase showed high residual activity at broad pH and temperature ranges. Using birchwood xylan as a substrate, the and values of xylanase nanoflowers were 1.60 mg/ml and 455 μmol/min/mg protein, compared with 1.42 mg/ml and 300 μmol/min/mg protein, respectively, for the free enzyme. After 5 and 10 cycles of reuse, the xylanase nanoflowers retained 87.5% and 75.8% residual activity, respectively. These results demonstrate that xylanase immobilization using a protein-inorganic hybrid nanoflower system is an effective approach for its potential biotechnological applications.
在本研究中，评估了使用蛋白质-无机杂化纳米花系统固定木聚糖酶以改善酶的性质。杂化木聚糖酶纳米花的合成在4°C下使用0.25 mg/ml蛋白质进行72小时非常有效，并且观察到木聚糖酶的有效固定，最大包封率和相对活性分别为78.5%和148%。固定化木聚糖酶在较宽的pH和温度范围内显示出高残留活性。以桦木木聚糖为底物，木聚糖酶纳米花的Km和Vmax值分别为1.60 mg/ml和455 μmol/min/mg蛋白质，而游离酶的Km和Vmax值分别为1.42 mg/ml和300 μmol/min/mg蛋白质。在重复使用5次和10次后，木聚糖酶纳米花分别保留了87.5%和75.8%的残留活性。这些结果表明，使用蛋白质-无机杂化纳米花系统固定木聚糖酶是其潜在生物技术应用的有效方法。
