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固定化马来酸酐改性木聚糖酶的热力学和物理化学性质及其在从麦麸中提取低聚糖中的应用

Thermodynamics and Physicochemical Properties of Immobilized Maleic Anhydride-Modified Xylanase and Its Application in the Extraction of Oligosaccharides from Wheat Bran.

作者信息

Zhao Yang, Li Xinrui, Guo Shuo, Xu Jingwen, Cui Yan, Zheng Mingzhu, Liu Jingsheng

机构信息

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.

National Engineering Research Center for Wheat and Corn Deep Processing, Changchun 130118, China.

出版信息

Foods. 2023 Jun 20;12(12):2424. doi: 10.3390/foods12122424.

DOI:10.3390/foods12122424
PMID:37372634
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10297198/
Abstract

Xylanases are the preferred enzymes for the extracting of oligosaccharides from wheat bran. However, free xylanases have poor stability and are difficult to reuse, which limit their industrial application. In the present study, we covalently immobilized free maleic anhydride-modified xylanase (FMA-XY) to improve its reusability and stability. The immobilized maleic anhydride-modified xylanase (IMA-XY) exhibited better stability compared with the free enzyme. After six repeated uses, 52.24% of the activity of the immobilized enzyme remained. The wheat bran oligosaccharides extracted using IMA-XY were mainly xylopentoses, xylohexoses, and xyloheptoses, which were the β-configurational units and α-configurational units of xylose. The oligosaccharides also exhibited good antioxidant properties. The results indicated that FMA-XY can easily be recycled and can remain stable after immobilization; therefore, it has good prospects for future industrial applications.

摘要

木聚糖酶是从小麦麸皮中提取低聚糖的首选酶。然而，游离木聚糖酶稳定性差且难以重复使用，这限制了它们的工业应用。在本研究中，我们通过共价固定游离的马来酸酐修饰木聚糖酶（FMA-XY）来提高其可重复使用性和稳定性。与游离酶相比，固定化马来酸酐修饰木聚糖酶（IMA-XY）表现出更好的稳定性。经过六次重复使用后，固定化酶仍保留52.24%的活性。使用IMA-XY提取的小麦麸皮低聚糖主要是木戊糖、木己糖和木庚糖，它们是木糖的β构型单元和α构型单元。这些低聚糖还表现出良好的抗氧化性能。结果表明，FMA-XY固定化后易于回收且能保持稳定；因此，它在未来工业应用中具有良好的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/9ae0da0337d7/foods-12-02424-g015a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/36e5eaa84c2e/foods-12-02424-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/758bb4a18347/foods-12-02424-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/a69bb732c958/foods-12-02424-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/77e0bba77f01/foods-12-02424-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/e837a728eb72/foods-12-02424-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/f7112e1a1b88/foods-12-02424-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/9ae0da0337d7/foods-12-02424-g015a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/36e5eaa84c2e/foods-12-02424-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/758bb4a18347/foods-12-02424-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/a69bb732c958/foods-12-02424-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/77e0bba77f01/foods-12-02424-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/e837a728eb72/foods-12-02424-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/f7112e1a1b88/foods-12-02424-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c39/10297198/9ae0da0337d7/foods-12-02424-g015a.jpg

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本文引用的文献

[1]
A multifaceted enzyme conspicuous in fruit juice clarification: An elaborate review on xylanase.

Int J Biol Macromol. 2021-12-15

[2]
In situ purification and enrichment of fructo-oligosaccharides by fermentative treatment with Bacillus coagulans and selective catalysis using immobilized fructosyltransferase.

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[3]
Structural characterization and antioxidant activity of oligosaccharides from Panax ginseng C. A. Meyer.

Int J Biol Macromol. 2020-2-4

[4]
Improving laccase activity and stability by HKUST-1 with cofactor via one-pot encapsulation and its application for degradation of bisphenol A.

J Hazard Mater. 2019-8-31

[5]
Immobilization of xylanase on modified grafted alginate polyethyleneimine bead based on impact of sodium cation effect.

Int J Biol Macromol. 2019-8-26

[6]
Investigating the Effect of Two-Point Surface Attachment on Enzyme Stability and Activity.

J Am Chem Soc. 2018-11-19

[7]
Improving the retention and reusability of Alpha-amylase by immobilization in nanoporous polyacrylamide-graphene oxide nanocomposites.

Int J Biol Macromol. 2018-9-13

[8]
A tri-enzyme co-immobilized magnetic complex: Process details, kinetics, thermodynamics and applications.

Int J Biol Macromol. 2018-7-9

[9]
Cross-linked enzyme aggregates of alginate lyase: A systematic engineered approach to controlled degradation of alginate hydrogel.

Int J Biol Macromol. 2018-3-22

[10]
Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.

Food Chem. 2018-7-1

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