穿心莲内酯缓解 spared nerve injury 模型小鼠病理性疼痛。

CONTEXT: Andrographolide (Andro), found in large quantities in Andrographis paniculata Nees (Acanthaceae), is anti-inflammatory, especially in the central nervous system (CNS) glia. OBJECTIVE: The objective of this study is to test Andro's ability to reduce allodynia in a spared nerve injury model. MATERIAL AND METHODS: Male 30 g BalbC mice were divided into four groups: (1) Sham-operated control (Sham-group); (2) nerve injured and treated with saline (Saline-group); (3) nerve injured and treated with Andro (Andro-group); (4) nerve injured and treated with non-steroidal anti-inflammatory drugs (NSAIDS) (NSAIDS-group). Andro or NSAIDS (diclofenac salt) were injected intraperitoneally at 5 mg/kg body weight daily. Mechanical allodynia was assessed by von Frey tests at 3, 7, and 14 d. For immunohistochemical analysis, samples were collected at 7 d. RESULTS: The threshold for inducing allodynia increased and the response percentage reduced in the Andro-group when compared with the Saline-group, as well as when compared with NSAIDS groups throughout 3-14 d. The ratio of threshold for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS groups was 20.42 and 11.67 at 14 d, respectively. The ratio of response percentage for OP-Andro/OP-saline and for OP-Andro/OP-NSAIDS was 0.32 and 0.39 at 14 d, respectively. Interleukin-1 (IL-1) immunostaining in the spinal cord was reduced in the Andro-group. Astrocytic activities were not significantly reduced in the Andro-group compared with the Saline-group at 7 d post-operation (PO) Conclusions: Andro reduced mechanical allodynia more than NSAIDS at the same concentration, and the observed behaviour was associated with a reduction in inflammatory cytokine produced in the spinal cord.

背景：穿心莲内酯（Andro）大量存在于穿心莲（爵床科）中，具有抗炎作用，尤其在中枢神经系统（CNS）胶质细胞中。

目的：本研究旨在测试穿心莲内酯降低 spared 神经损伤模型中痛觉过敏的能力。

材料和方法：雄性 30g BalbC 小鼠分为四组：（1）假手术对照（Sham 组）；（2）神经损伤并用生理盐水处理（Saline 组）；（3）神经损伤并用穿心莲内酯处理（Andro 组）；（4）神经损伤并用非甾体抗炎药（NSAIDs）处理（NSAIDs 组）。Andro 或 NSAIDs（双氯芬酸钠盐）以 5mg/kg 体重每天腹腔注射。通过 von Frey 试验在 3、7 和 14d 评估机械性痛觉过敏。为了进行免疫组织化学分析，在 7d 时采集样本。

结果：与 Saline 组相比，Andro 组诱导痛觉过敏的阈值增加，反应百分比降低，与 NSAIDs 组在 3-14d 期间相比也是如此。在 14d 时，OP-Andro/OP-saline 和 OP-Andro/OP-NSAIDs 组的阈值比分别为 20.42 和 11.67。在 14d 时，OP-Andro/OP-saline 和 OP-Andro/OP-NSAIDs 组的反应百分比比分别为 0.32 和 0.39。在脊髓中，白细胞介素-1（IL-1）免疫染色在 Andro 组中减少。与 Saline 组相比，在手术后 7d（PO）时，Andro 组的星形胶质细胞活性没有明显降低。

结论：与相同浓度的 NSAIDs 相比，Andro 更能减轻机械性痛觉过敏，观察到的行为与脊髓中产生的炎症细胞因子减少有关。