Kamal Aisha, Saify Zafar Saeed, Jamali Khawar Saeed, Tufail Priya, Kanwal Aiman, Khan Faisal, Siddiqui Sonia
Department of Neuroscience, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
HEJ research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
Pak J Pharm Sci. 2018 Jan;31(1(Suppl.)):257-261.
In view of the well-documented medicinal properties of Calotropis procera (CP), the present study was designed to evaluate the neuroprotective effect of the extract. We have prepared a methanolic extract of Calotropis procera and screen varying concentration of CP (20, 30, 40, 50 and 70μg/ml) for the stimulatory potency on neurite outgrowth. The stimulatory effect of CP on neurite outgrowth was assessed in primary hippocampal neurons. Neurite lengths were measured using optika provison analysis software. Neuritogenesis was further analyzed by immunostaining by using specific neuronal marker β III-tubulin. The data show that neurite outgrowth from hippocampal neurons were significantly enhanced in the presence of CP (40μg/ml). The most stimulatory neurite outgrowth effects were appeared after 48hrs incubation of neurons with CP (40μg/ml). These data confirm that CP extract could promote invitro hippocampal neurite outgrowth in a dose-dependent manner. Our results indicate that CP can be used as a healthy dietary supplement for the cognitive functions of the brain.
鉴于牛角瓜（CP）具有充分记载的药用特性，本研究旨在评估其提取物的神经保护作用。我们制备了牛角瓜的甲醇提取物，并筛选了不同浓度的CP（20、30、40、50和70μg/ml）对神经突生长的刺激效力。在原代海马神经元中评估CP对神经突生长的刺激作用。使用Optika Provision分析软件测量神经突长度。通过使用特异性神经元标志物β III-微管蛋白进行免疫染色进一步分析神经发生。数据显示，在存在CP（40μg/ml）的情况下，海马神经元的神经突生长显著增强。在用CP（40μg/ml）孵育神经元48小时后，出现了最强烈的神经突生长刺激作用。这些数据证实，CP提取物可以以剂量依赖的方式促进体外海马神经突生长。我们的结果表明，CP可作为一种健康的膳食补充剂用于大脑的认知功能。
