Supino R, Della Torre G, Ramponi R, Bottiroli G
Chem Biol Interact. 1986 Mar;57(3):285-94. doi: 10.1016/0009-2797(86)90003-7.
This paper concerns a general study on the effects of hematoporphyrin-derivative (HpD) on mouse erythroleukemia (MEL) cells, in the absence of light irradiation. In particular, HpD intrinsic cytotoxicity was evaluated at different doses and the results correlated with those referring to membrane functional and morphological changes. HpD uptake and release processes were also studied and compared with the above-mentioned results. In order to have an overall picture of HpD-cell interactions, time-resolved fluorescence measurements were performed on both undifferentiated and differentiated MEL cells. The results obtained indicate that, even at HpD doses exhibiting neither any cytotoxicity nor any morphological damage (1-10 micrograms/ml), membrane permeability alterations are observed. Thirty minutes of treatment are sufficient for HpD to develop its toxic effect: indeed, no differences in HpD influence on cell viability can be observed after 30 min, 60 min or 5 days of treatment. HpD cytotoxicity is reduced by high protein content in the incubation culture medium. The presence of both monomeric species and 580 nm emitting species was observed at cellular level. The latter is likelier in undifferentiated MEL cells, which also exhibit higher overall HpD uptake, as compared with differentiated MEL cells.
本文是关于在无光照情况下血卟啉衍生物（HpD）对小鼠红白血病（MEL）细胞作用的一项综合研究。特别地，评估了不同剂量下HpD的内在细胞毒性，并将结果与膜功能和形态变化相关联。还研究了HpD的摄取和释放过程，并与上述结果进行比较。为全面了解HpD与细胞的相互作用，对未分化和分化的MEL细胞进行了时间分辨荧光测量。所得结果表明，即使在HpD剂量既未表现出任何细胞毒性也未造成任何形态损伤（1 - 10微克/毫升）时，也能观察到膜通透性改变。30分钟的处理足以使HpD发挥其毒性作用：实际上，处理30分钟、60分钟或5天后，HpD对细胞活力的影响没有差异。在孵育培养基中高蛋白含量会降低HpD的细胞毒性。在细胞水平观察到单体形式和发射波长为580纳米的物质均存在。与分化的MEL细胞相比，后者在未分化的MEL细胞中更常见，未分化的MEL细胞总体上对HpD的摄取也更高。
