用于检测卵巢癌中人类附睾蛋白4的基于磁性颗粒的化学发光免疫分析方法的制备
The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer.
作者信息
Fu Xiaoling, Liu Yangyang, Qiu Ruiyun, Foda Mohamed F, Zhang Yong, Wang Tao, Li Jinshan
机构信息
Department of Orthopedic, The 2nd Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, PR China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
出版信息
Biochem Biophys Rep. 2018 Jan 11;13:73-77. doi: 10.1016/j.bbrep.2018.01.002. eCollection 2018 Mar.
The magnetic particles have a significant influence on the immunoassay detection and cancer therapy. Herein, the chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) was presented for the clinical determination and analysis of human epididymis protein 4 (HE4) in the human serum. Under the optimized experiment conditions, the secure MPCLIA method can detect HE4 in the broader range of 0-1000 pmol/L, with a lower detection limit of 1.35 pmol/L. The satisfactory recovery rate of the method in the serum ranged from 83.62% to 105.10%, which was well within the requirement of clinical analysis. Moreover, the results showed the good correlation with enzyme-linked immunosorbent assay (ELISA), with the correlation coefficient of 0.9589. This proposed method has been successfully applied to the clinical determination of HE4 in the human serum.
磁性颗粒对免疫分析检测和癌症治疗有显著影响。在此，提出了一种结合磁性颗粒的化学发光免疫分析方法（MPCLIA），用于临床测定和分析人血清中的人附睾蛋白4（HE4）。在优化的实验条件下，安全的MPCLIA方法能够在0 - 1000 pmol/L的较宽范围内检测HE4，检测下限为1.35 pmol/L。该方法在血清中的回收率令人满意，范围为83.62%至105.10%，完全符合临床分析要求。此外，结果显示与酶联免疫吸附测定法（ELISA）具有良好的相关性，相关系数为0.9589。该方法已成功应用于人血清中HE4的临床测定。
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