Salarkia Ehsan, Sepehri Gholamreza, Torabzadeh Parvin, Abshenas Jalil, Saberi Arezoo
Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Int J Reprod Biomed. 2017 Oct;15(10):625-634.
Male infertility has been reported following long-term sulfasalazine, however, the precise effects of co-trimoxazole on sperm quality is controversial.
In this study, we evaluated the effects of co-trimoxazole and its co-administration with folic acid on sperm quality and histological changes of testes in male rats.
In this experimental study, 136 male Wistar rats were divided into 9 groups: I (control), II (vehicle) received saline, III: received folic acid (1 mg/kg /daily i.p., and IV- IX received co-trimoxazole (30, 60, and 120 mg/kg/daily; i.p.)+folic acid (1 mg/kg/daily; i.p.) for 14 or 28 days. Sperm samples were obtained from each group at the end of 14 and 28 days. Sperm numbers, motility, and viability were evaluated on a hemocytometer. Hematoxylin and Eosin stained testes were done for evaluation ofthe number of Leydig cells, vascularity, spermatids, spermatocytes, and means of seminiferous tubules diameter under light microscopy.
Co-trimoxazole treatment for either 14 or 28 days caused a significant decrease in the percentage of sperm number, motility, and viability (p<0.001) compared to the control group. Also, high doses of co-trimoxazole caused a significant decrease in testes structural abnormalities means of seminiferous tubules diameter, spermatids, and spermatogonia) compared to the vehicle group (p<0.001). Folic acid co-administration with co-trimoxazole partially reversed the decrease in sperm quality and structural abnormalities of high doses of co-trimoxazole (60 and 120 mg/kg/daily) (p<0.001).
The data showed the adverse effects of co-trimoxazole on sperm quality and testes morphology which was protected partially by folic acid co-administration in rats. The underlying mechanism (s) needs further investigations.
长期服用柳氮磺胺吡啶后有男性不育的报道，然而，复方新诺明对精子质量的确切影响存在争议。
在本研究中，我们评估了复方新诺明及其与叶酸联合应用对雄性大鼠精子质量和睾丸组织学变化的影响。
在本实验研究中，136只雄性Wistar大鼠被分为9组：I组（对照组），II组（溶剂对照组）接受生理盐水，III组：接受叶酸（1毫克/千克/每日腹腔注射），IV - IX组接受复方新诺明（30、60和120毫克/千克/每日；腹腔注射）+叶酸（1毫克/千克/每日；腹腔注射），持续14天或28天。在14天和28天结束时从每组获取精子样本。在血细胞计数器上评估精子数量、活力和存活率。对苏木精和伊红染色的睾丸进行评估，以在光学显微镜下观察睾丸间质细胞数量、血管分布、精子细胞、精母细胞以及生精小管直径平均值。
与对照组相比，复方新诺明治疗14天或28天均导致精子数量、活力和存活率百分比显著降低（p<0.001）。此外，与溶剂对照组相比，高剂量的复方新诺明导致睾丸结构异常（生精小管直径、精子细胞和精原细胞）显著减少（p<0.001）。叶酸与复方新诺明联合应用部分逆转了高剂量复方新诺明（60和120毫克/千克/每日）导致的精子质量下降和结构异常（p<0.001）。
数据显示复方新诺明对精子质量和睾丸形态有不良影响，而在大鼠中叶酸联合应用可部分保护这些影响。其潜在机制需要进一步研究。
