Department of Physics, National Taiwan University, Taipei, Taiwan.
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.
J Biophotonics. 2018 Sep;11(9):e201700338. doi: 10.1002/jbio.201700338. Epub 2018 May 9.
In this study, intravital multiphoton microscopy was used to quantitatively investigate hepatobiliary metabolism in chronic pathologies of the liver. Specifically, through the use of the probe molecule 6-carboxyfluorescein diacetate, the effects of liver fibrosis, fatty liver, and hepatocellular carcinoma on the metabolic capabilities of mouse liver were investigated. After the acquisition of time-lapse images, a first order kinetic model was used to calculate rate constant resolved images of various pathologies. It was found that the ability of the liver to metabolically process the probe molecules varies among different pathologies, with liver fibrosis and fatty liver disease negatively impacted the uptake, processing, and excretion of molecules. The approach demonstrated in this work allows the study of the response of hepatic functions to different pathologies in real time and is useful for studying processes such as pharmacokinetics through direct optical imaging.
在这项研究中，我们使用活体多光子显微镜定量研究了肝脏慢性病变中的肝胆代谢。具体来说，通过使用探针分子 6-羧基荧光素二乙酸酯，研究了肝纤维化、脂肪肝和肝癌对小鼠肝脏代谢能力的影响。在采集时移图像后，使用一阶动力学模型计算了各种病变的速率常数分辨图像。结果发现，肝脏代谢探针分子的能力在不同病变中有所不同，肝纤维化和脂肪肝疾病会对分子的摄取、处理和排泄产生负面影响。本工作中展示的方法允许实时研究肝脏功能对不同病变的反应，并且通过直接光学成像，对于研究药代动力学等过程非常有用。
