通过在蔗糖梯度中进行区带速度离心分离的狒狒关节软骨蛋白聚糖单体的凝胶电泳。
Gel electrophoresis of proteoglycan monomers of baboon articular cartilage separated by zonal rate centrifugation in sucrose gradients.
作者信息
Stanescu V, Pham T D
出版信息
Connect Tissue Res. 1986;14(3):169-77. doi: 10.3109/03008208609014257.
PMID:2938876
Abstract
Proteoglycan monomers from articular cartilage of young baboons and of a baboon foetus were submitted to zonal rate centrifugation in sucrose gradients. The peaks obtained were analyzed by gel electrophoresis on polyacrylamide-agarose gels. The proteoglycan monomers corresponding to the bands obtained by gel-electrophoresis of unfractionated proteoglycans were separated in the gradients and the sedimentation distance correlated with the electrophoretic mobility.
摘要
将幼年狒狒和狒狒胎儿关节软骨中的蛋白聚糖单体在蔗糖梯度中进行区带速率离心。通过聚丙烯酰胺 - 琼脂糖凝胶电泳分析得到的峰。将对应于未分级蛋白聚糖凝胶电泳所得条带的蛋白聚糖单体在梯度中分离，并将沉降距离与电泳迁移率相关联。
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