Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 1 Nongda South Rd., Xi Beiwang, Haidian District, Beijing 100193, PR China.
Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-products Processing, Ministry of Agriculture, No. 1 Nongda South Rd., Xi Beiwang, Haidian District, Beijing 100193, PR China.
Food Res Int. 2018 Jan;103:156-162. doi: 10.1016/j.foodres.2017.10.023. Epub 2017 Oct 21.
Wheat, corn, tapioca, sweet potato and potato starches were independently mixed into starch-gluten model doughs containing 15% (w/w) vital gluten. Rheological properties, including linear viscoelasticity region, frequency dependence and recovery capacity, were studied by strain sweep, frequency sweep, and creep and recovery measurements. Structural properties were also investigated by measuring the disulfide bonds (-SS-) content, SDS-PAGE and low-resolution H nuclear magnetic resonance. Wheat starch (WS)-gluten dough had the greatest linear viscoelasticity region (0.190%), lowest frequency dependence (0.128) and greatest recovery capacity (67.39%), while potato starch-gluten dough had the smallest linear viscoelasticity region (0.126%), greatest frequency dependence (0.195) and lowest recovery capacity (54.97%). Furthermore, WS-gluten dough showed the highest disulfide bonds (-SS-) content (3.47μmol/g), lowest intensity of extracted glutenin bands and highest bond water content (23.20%). This suggested that WS-gluten dough formed stronger starch-gluten interactions compared with those of the other four starch-gluten model doughs.
小麦、玉米、木薯、甘薯和马铃薯淀粉分别与含 15%(w/w)活性面筋的面筋-淀粉模型面团混合。通过应变扫描、频率扫描、蠕变和恢复测量研究了流变性质,包括线性粘弹性区、频率依赖性和恢复能力。还通过测量二硫键(-SS-)含量、SDS-PAGE 和低分辨率 H 核磁共振研究了结构性质。小麦淀粉(WS)-面筋面团具有最大的线性粘弹性区(0.190%)、最低的频率依赖性(0.128)和最大的恢复能力(67.39%),而马铃薯淀粉-面筋面团具有最小的线性粘弹性区(0.126%)、最大的频率依赖性(0.195)和最低的恢复能力(54.97%)。此外,WS-面筋面团表现出最高的二硫键(-SS-)含量(3.47μmol/g)、最低的提取谷蛋白带强度和最高的结合水含量(23.20%)。这表明与其他四种淀粉-面筋模型面团相比,WS-面筋面团形成了更强的淀粉-面筋相互作用。