Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Blue Marble Space Institute of Science, Seattle, WA 98154, USA.
FEMS Microbiol Ecol. 2018 Mar 1;94(3). doi: 10.1093/femsec/fiy014.
DNA stable isotope probing (SIP) was used to track the uptake of organic and inorganic carbon sources for TACK archaea (Thaumarchaeota/Aigarchaeota/Crenarchaeota/Korarchaeota) on a cruise of opportunity in the North Atlantic. Due to water limitations, duplicate samples from the deep photic (60-115 m), the mesopelagic zones (local oxygen minimum; 215-835 m) and the bathypelagic zone (2085-2835 m) were amended with various combinations of 12C- or 13C-acetate/urea/bicarbonate to assess cellular carbon acquisition. The SIP results indicated the majority of TACK archaeal operational taxonomic units (OTUs) incorporated 13C from acetate and/or urea into newly synthesized DNA within 48 h. A small fraction (16%) of the OTUs, often representing the most dominant members of the archaeal community, were able to incorporate bicarbonate in addition to organic substrates. Only two TACK archaeal OTUs were found to incorporate bicarbonate but not urea or acetate. These results further demonstrate the utility of SIP to elucidate the metabolic capability of mesothermal archaea in distinct oceanic settings and suggest that TACK archaea play a role in organic carbon recycling in the mid-depth to deep ocean.
DNA 稳定同位素探测 (SIP) 被用于追踪北大西洋一次偶然巡航中 TACK 古菌（泉古菌门/广古菌门/奇古菌门/古菌门）对有机和无机碳源的摄取。由于水资源限制，从深光区（60-115 米）、中层带（局部氧最小值；215-835 米）和深海带（2085-2835 米）采集的重复样本中添加了各种 12C-或 13C-醋酸盐/尿素/碳酸氢盐组合，以评估细胞对碳的获取。SIP 结果表明，大多数 TACK 古菌的操作分类单元（OTU）在 48 小时内将醋酸盐和/或尿素中的 13C 掺入新合成的 DNA 中。一小部分（16%）的 OTU，通常代表古菌群落中最主要的成员，除了有机底物外，还能够掺入碳酸氢盐。只有两个 TACK 古菌 OTU 被发现能够掺入碳酸氢盐而不是尿素或醋酸盐。这些结果进一步证明了 SIP 用于阐明不同海洋环境中中温古菌代谢能力的有效性，并表明 TACK 古菌在中深至深海的有机碳循环中发挥作用。
