a Department of Biology, Damghan Branch , Islamic Azad University , Damghan , Iran.
b Department of Pharmacology, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
Arch Physiol Biochem. 2019 Feb;125(1):25-29. doi: 10.1080/13813455.2018.1430158. Epub 2018 Feb 1.
The aim of the present experiment was to investigate the possible antiarrhythmic effects of dalfampridine in ouabain-induced arrhythmia in rats. Twenty-four male rats including the control and dalfampridine-incubated (100 µM to 10 mM) ouabain-stimulated (40 µM) groups were used. After induction of anesthesia, the atria were isolated and the time of onset of arrhythmia and asystole were recorded. The contractile force of atria was also measured. Dalfampridine at concentration of 1 mM significantly postponed the onset of arrhythmia and asystole compared to control group (p ≤ .05). Ouabain significantly increased the atrial beating rate in control group (p ≤ .05), while pretreatment of isolated atria with dalfampridine reversed this effect. Incubation of isolated atria with ouabain did not alter the contractile force in both control- and dalfampridine-treated groups (p > .05). It is concluded that dalfampridine might possess antiarrhythmic properties in reducing the atrial arrhythmias.
本实验旨在研究地夫可特在哇巴因诱导的大鼠心律失常中的可能抗心律失常作用。使用了 24 只雄性大鼠，包括对照组和地夫可特孵育组（100µM 至 10mM）以及哇巴因刺激组（40µM）。在诱导麻醉后，分离心房并记录心律失常和停搏的发作时间。还测量了心房的收缩力。与对照组相比，浓度为 1mM 的地夫可特明显延迟了心律失常和停搏的发作（p≤.05）。哇巴因可显著增加对照组的心房跳动率（p≤.05），而用地夫可特预处理分离的心房可逆转这种作用。在对照组和地夫可特处理组中，孵育分离的心房用哇巴因不会改变收缩力（p>.05）。结论是，地夫可特可能具有抗心律失常特性，可减少心房性心律失常。
