Gowande Gaurang G, Tembe Sanket, Ghate Hemant V
a Department of Biotechnology , Fergusson College , Pune , India.
b Department of Zoology , Modern College , Pune , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Dec;29(8):1215-1223. doi: 10.1080/24701394.2018.1431229. Epub 2018 Feb 1.
Cytochrome c oxidase subunit I gene (COI) sequences of roughly 509 bp length for various species of the Infraorder Pentatomomorpha were generated. K2P divergences within and between species and genera were calculated and compared using newly generated sequences and the ones available on online portals. Mean interspecific (within-genus) genetic divergence (14.23%) was ∼ eight times greater than mean intraspecific (within-species) divergence (1.79%). Distance-based as well as character-based approaches were used towards constructing (COI) trees. In total, 20 sequences were of the species that were previously not part of the Barcode Of Life Database (BOLD), hence representing additions to the barcode library of Indian Heteroptera. Some of the analyzed species are well-known agricultural pests. All the COI sequences and the associated specimen data have been deposited on BOLD.
生成了半翅目异翅亚目各种物种长度约为509 bp的细胞色素c氧化酶亚基I基因（COI）序列。使用新生成的序列以及在线平台上可获得的序列，计算并比较了物种内和属间的K2P分歧。种间（属内）平均遗传分歧（14.23%）约为种内（种内）平均分歧（1.79%）的八倍。基于距离和基于特征的方法被用于构建（COI）树。总共有20个序列来自以前不属于生命条形码数据库（BOLD）的物种，因此是印度异翅亚目条形码库的新增内容。一些分析的物种是著名的农业害虫。所有的COI序列和相关标本数据都已存入BOLD。
