胃癌组织中Th17/Treg相关分子的表达
Expression of Th17/Treg related molecules in gastric cancer tissues.
作者信息
Meng Xinying, Zhu Shengtao, Dong Quanjiang, Zhang Shutian, Ma Jian, Zhou Changhong
机构信息
Department of Health Care, Qingdao Municipal Hospital (East), Qingdao, China.
Beijing Digestive Diseases Center, Beijing Friendship Hospital, Beijing, China; Beijing Key Laboratory for Precancerous Lesion of Digestive Diseases, Beijing, China.
出版信息
Turk J Gastroenterol. 2018 Jan;29(1):45-51. doi: 10.5152/tjg.2018.17114.
BACKGROUND/AIMS: The function of regulatory T cells (Treg) and helper T cells 17 (Th17) related indexes, such as interleukin (IL)-6, IL-17, transforming growth factor (TGF)-β1, and forkhead box protein 3(FoxP3) in gastric adenocarcinoma tissues remains undefined. We investigated and analyzed the relevance of the proteins with the clinicopathological characteristics and the interactions among them in gastric cancer.
MATERIALS AND METHODS
A total 68 gastric cancer patients and 40 healthy controls were enrolled. Immunohistochemistry (IHC) as well as quantitative real-time reverse transcription- polymerase chain reaction (RT-PCR) was used to determine the expression levels of IL-6, TGF-β1, IL-17, and FoxP3 in the prepared tissues. Statistical analysis included ANOVA and chi-square test.
RESULTS
The expression levels of IL-6, IL-17, FoxP3, and TGF-β1 had significantly increased in cancer tissues compared to controls. Clinical staging of gastric cancer were correlated with the rise of IL-6, IL-17, FoxP3, and TGF-β1 levels expressed in cancer tissues. The expression level of TGF-β1 and IL-6 was positively related to that of IL-17 and FoxP3, similar to FoxP3 and IL-17 in gastric cancer tissues.
CONCLUSION
IL-6, TGF-β1, FoxP3, and IL-17 may promote the progression of gastric cancer individually or jointly and have complex interactions.
背景/目的：胃腺癌组织中调节性T细胞（Treg）和辅助性T细胞17（Th17）相关指标，如白细胞介素（IL）-6、IL-17、转化生长因子（TGF）-β1和叉头框蛋白3（FoxP3）的功能仍不明确。我们研究并分析了这些蛋白与胃癌临床病理特征的相关性以及它们之间的相互作用。
材料与方法
共纳入68例胃癌患者和40例健康对照。采用免疫组织化学（IHC）以及定量实时逆转录-聚合酶链反应（RT-PCR）来测定所制备组织中IL-6、TGF-β1、IL-17和FoxP3的表达水平。统计分析包括方差分析和卡方检验。
结果
与对照组相比，癌组织中IL-6、IL-17、FoxP3和TGF-β1的表达水平显著升高。胃癌的临床分期与癌组织中IL-6、IL-17、FoxP3和TGF-β1水平的升高相关。TGF-β1和IL-6的表达水平与IL-17和FoxP3的表达水平呈正相关，胃癌组织中FoxP3和IL-17的情况类似。
结论
IL-6、TGF-β1、FoxP3和IL-17可能单独或共同促进胃癌进展并存在复杂的相互作用。
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