Amirrasouli Muhammad Mehdi, Shamsara Mehdi
Department of Cardiovascular Medicine, Institute of Genetic Medicine, International centre for life, School of Medicine, Newcastle University, UK.
National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e- Pajoohesh, 15th Km, Tehran -Karaj Highway, Tehran, Iran.
J Stem Cells Regen Med. 2017 Dec 18;13(2):35-44. doi: 10.46582/jsrm.1302007. eCollection 2017.
Coronary heart disease (CHD) is still one of the main causes of death in the world, despite significant advances in clinical treatments. Stem cell transplantation methods have the potential to improve cardiac function and patients' outcome following heart attack, but optimal cell types, cell preparation methods and cell delivery routes are yet to be developed. Mammalian hearts contain a small fraction of progenitor cells which, in culture, migrate out of the cardiac explants, known as explant-derived cell (EDCs) and contribute to spheroids known as cardiospheres (Csphs). Following further culture and cell passaging, Csphs give rise to cardiosphere-derived cells (CDCs). EDCs, Csphs and CDCs show and angiogenesis and tissue regeneration in myocardial ischemia. However, CDC and Csph formation is time consuming, expensive and not always successful. Therefore, this study aims to compare EDCs with CDCs and assess the effect of hypoxic preconditioning on their pro-angiogenic potential. The data showed that preconditioning EDCs in hypoxic cell culture enhances cell growth, viability and expression of stem cell and pro-angiogenic markers more than CDCs. experiments using a sub-dermal matrigel plug assay showed that EDCs and CDCs alone have limited pro-angiogenic potential; however, hypoxic preconditioning of EDCs and CDCs significantly enhances this process. Further research will increase our understanding of cardiac stem cell mediated angiogenesis and improve clinical therapies for myocardial infarction (MI) patients.
尽管临床治疗取得了重大进展，但冠心病（CHD）仍是世界上主要的死亡原因之一。干细胞移植方法有可能改善心脏病发作后的心脏功能和患者预后，但最佳的细胞类型、细胞制备方法和细胞递送途径仍有待开发。哺乳动物心脏含有一小部分祖细胞，在培养过程中，这些祖细胞会从心脏外植体中迁移出来，称为外植体衍生细胞（EDCs），并形成称为心肌球（Csphs）的球体。经过进一步培养和细胞传代后，心肌球产生心肌球衍生细胞（CDCs）。EDCs、Csphs和CDCs在心肌缺血中显示出血管生成和组织再生能力。然而，CDC和Csph的形成既耗时又昂贵，而且并不总是成功。因此，本研究旨在比较EDCs和CDCs，并评估缺氧预处理对其促血管生成潜力的影响。数据显示，在缺氧细胞培养中对EDCs进行预处理比CDCs更能促进细胞生长、活力以及干细胞和促血管生成标志物的表达。使用皮下基质胶塞试验的实验表明，单独的EDCs和CDCs促血管生成潜力有限；然而，对EDCs和CDCs进行缺氧预处理可显著增强这一过程。进一步的研究将增进我们对心脏干细胞介导的血管生成的理解，并改善对心肌梗死（MI）患者的临床治疗。
