van der Meer Jitse M
Department of Zoology, Radboud University, Huygensgebouw, Heyendaalseweg, Nijmegen, The Netherlands.
Department of Zoology, University of Heidelberg, Im Neuenheimer Feld, Heidelberg, Germany.
J Exp Zool B Mol Dev Evol. 2018 Jan;330(1):52-59. doi: 10.1002/jez.b.22789. Epub 2018 Feb 2.
The genetic regulation of anterior-posterior segment pattern development has been elucidated in detail for Drosophila, but it is not canonical for insects. A surprising diversity of regulatory mechanisms is being uncovered not only between insect orders, but also within the order of the Diptera. The question is whether the same diversity of regulatory mechanisms exists within other insect orders. I show that anterior puncture of the egg of the pea beetle Callosobruchus maculatus submerged in RNase can induce double abdomen development suggesting a role for maternal mRNA. In a double abdomen, anterior segments are replaced by posterior segments oriented in mirror image symmetry to the original posterior segments. This effect is specific for RNase activity, for treatment of the anterior egg pole and for cytoplasmic RNA. Yield depends on developmental stage, enzyme concentration, and temperature. A maximum of 30% of treated eggs reversed segment sequence after submersion and puncture in 10 μg/mL RNase S reconstituted from S-protein and S-peptide at 30°C. This result sets the stage for an analysis of the genetic regulation of segment pattern formation in the long germ embryo of the coleopteran Callosobruchus and for comparison with the short germ embryo of the coleopteran Tribolium.
果蝇前后体节模式发育的遗传调控已得到详细阐明，但这并非昆虫的典型模式。不仅在不同昆虫目之间，而且在双翅目内部，都发现了令人惊讶的调控机制多样性。问题在于其他昆虫目是否也存在同样多样的调控机制。我发现，将黄斑豆象卵浸于核糖核酸酶中并在前部穿刺，可诱导双腹发育，这表明母体信使核糖核酸发挥了作用。在双腹中，前部体节被后部体节取代，且与原始后部体节呈镜像对称排列。这种效应对于核糖核酸酶活性、卵前部的处理以及细胞质核糖核酸具有特异性。产量取决于发育阶段、酶浓度和温度。在30°C下，将卵浸于由S蛋白和S肽重构的10μg/mL核糖核酸酶S中并穿刺后，最多30%的处理卵出现了体节序列反转。这一结果为分析鞘翅目黄斑豆象长胚胚胎中体节模式形成的遗传调控以及与鞘翅目赤拟谷盗短胚胚胎进行比较奠定了基础。
