Burns R A, Klaunig J E, Shulok J R, Davis W J, Goldblatt P J
Oral Surg Oral Med Oral Pathol. 1986 Apr;61(4):368-72. doi: 10.1016/0030-4220(86)90421-4.
The tumor-localizing and photochemotherapeutic properties of hematoporphyrin derivative (HPD) were examined in 7, 12 dimethylbenzanthracene (DMBA)-induced oral cancers in the Syrian hamster. Oral tumors in hamsters injected with HPD (50 micrograms per gram of body weight) exhibited bright salmon pink fluorescence when exposed to long-wave ultraviolet light 24 hours after intraperitoneal HPD injection. Adjacent tumor-free mucosa did not fluoresce. Similarly, tumors not treated with HPD, normal mucosa treated with HPD, and normal mucosa not treated with HPD did not fluoresce. Tumors in animals that received HPD and photochemotherapy (PCT) were examined for gross and microscopic pathologic changes following the phototreatment. Tumors displayed edema, hemorrhage, and cellular necrosis that progressed with the time of sampling after photochemotherapy. Complete tumor necrosis was evident in the majority of oral tumors 24 hours after HPD PCT.
在叙利亚仓鼠中，对血卟啉衍生物（HPD）的肿瘤定位和光化学治疗特性进行了研究，这些仓鼠患有由7,12 - 二甲基苯并蒽（DMBA）诱发的口腔癌。腹腔注射HPD（每克体重50微克）后24小时，当暴露于长波紫外线下时，注射了HPD的仓鼠口腔肿瘤呈现出明亮的鲑鱼粉红色荧光。相邻的无肿瘤黏膜不发荧光。同样，未用HPD治疗的肿瘤、用HPD治疗的正常黏膜以及未用HPD治疗的正常黏膜均不发荧光。对接受HPD和光化学疗法（PCT）的动物的肿瘤进行了光疗后大体和显微镜下病理变化检查。肿瘤表现出水肿、出血和细胞坏死，这些变化在光化学疗法后的取样时间内不断进展。在HPD - PCT后24小时，大多数口腔肿瘤出现明显的完全肿瘤坏死。
