Liao Chenlong, Yang Min, Liu Pengfei, Zhong Wenxiang, Zhang Wenchuan
Department of Neurosurgery, XinHua Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, Republic of China.
J Reconstr Microsurg. 2018 May;34(4):264-269. doi: 10.1055/s-0037-1621723. Epub 2018 Feb 2.
Preclinical studies involving animal models are essential for understanding the underlying mechanisms of diabetic neuropathic pain.
Rats were divided into four groups: two controls and two experimental. Diabetes mellitus was induced by streptozotocin (STZ) injection in two experimental groups. The first group involved one sham operation. The second group involved one latex tube encircling the sciatic nerve. The vehicle-injection rats were used as two corresponding control groups: sham operation and encircled nerves. By the third week, STZ-injected rats with encircled nerves were further divided into three subgroups: one involving continuing observation and the other two involving decompression (removal of the latex tube) at different time points (third week and fifth week). Weight and blood glucose were monitored, and behavioral analysis, including paw withdrawal threshold (PWT) and latency, was performed every week during the experimental period (7 weeks).
Hyperglycemia was induced in all STZ-injected rats. A significant increase in weight was observed in the control groups when compared with the experimental groups. By the third week, more STZ-injected rats with encircled nerves developed mechanical allodynia than those without (< 0.05), while no significant difference was noted (> 0.05) on the incidence of thermal hyperalgesia. Mechanical allodynia, but not thermal hyperalgesia, could be ameliorated by the removal of the latex tube at an early stage (third week).
With the combined use of a latex tube and STZ injection, a stable rat model of painful diabetic peripheral neuropathy (DPN) manifesting both thermal hyperalgesia and mechanical allodynia has been established.
涉及动物模型的临床前研究对于理解糖尿病性神经病理性疼痛的潜在机制至关重要。
将大鼠分为四组：两个对照组和两个实验组。在两个实验组中通过注射链脲佐菌素（STZ）诱导糖尿病。第一组进行一次假手术。第二组用一根乳胶管环绕坐骨神经。注射溶媒的大鼠用作两个相应的对照组：假手术组和神经环绕组。到第三周时，神经被环绕的STZ注射大鼠进一步分为三个亚组：一个亚组继续观察，另外两个亚组在不同时间点（第三周和第五周）进行减压（移除乳胶管）。在实验期间（7周）每周监测体重和血糖，并进行行为分析，包括爪部退缩阈值（PWT）和潜伏期。
所有注射STZ的大鼠均出现高血糖。与实验组相比，对照组体重显著增加。到第三周时，神经被环绕的STZ注射大鼠中出现机械性异常性疼痛的比未被环绕的更多（<0.05），而在热痛觉过敏发生率方面未观察到显著差异（>0.05）。早期（第三周）移除乳胶管可改善机械性异常性疼痛，但不能改善热痛觉过敏。
通过联合使用乳胶管和注射STZ，建立了一种稳定的表现出热痛觉过敏和机械性异常性疼痛的糖尿病性周围神经病理性疼痛（DPN）大鼠模型。
