多发性硬化症大鼠模型腰椎脊髓胆碱能失衡。
Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis.
机构信息
Department of Radiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Department of Radiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
出版信息
J Neuroimmunol. 2018 May 15;318:29-35. doi: 10.1016/j.jneuroim.2018.01.016. Epub 2018 Feb 12.
Abstract
Cholinergic dysfunction in the central nervous system is an important characteristic of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE). By using a rat EAE model, upregulation of vesicular acetylcholine transporter (VAChT) level in the EAE rat lumbar spinal cord was detected by western blot and immunostaining, and was associated with lymphocyte filtration and glial activation. Ex vivo and in vitro autoradiography studies with [F]VAT, a VAChT-specific radioligand, also revealed increased tracer uptake in EAE rat lumbar spinal cord compared with shams. These studies on VAChT expression suggest central cholinergic imbalance during EAE progression.
摘要
中枢胆碱能功能障碍是多发性硬化症和实验性自身免疫性脑脊髓炎（EAE）的一个重要特征。通过使用大鼠 EAE 模型，通过 Western blot 和免疫染色检测到 EAE 大鼠脊髓中的囊泡乙酰胆碱转运体（VAChT）水平上调，与淋巴细胞滤过和神经胶质激活有关。用 [F]VAT（一种 VAChT 特异性放射性配体）进行的离体和体外放射自显影研究也显示，与假手术组相比，EAE 大鼠脊髓中的示踪剂摄取增加。这些关于 VAChT 表达的研究表明，EAE 进展过程中存在中枢胆碱能失衡。
相似文献
1
Cholinergic imbalance in lumbar spinal cord of a rat model of multiple sclerosis.多发性硬化症大鼠模型腰椎脊髓胆碱能失衡。
J Neuroimmunol. 2018 May 15;318:29-35. doi: 10.1016/j.jneuroim.2018.01.016. Epub 2018 Feb 12.
2
Immunohistochemical studies on disabled-2 protein in the spinal cords of rats with experimental autoimmune encephalomyelitis.实验性自身免疫性脑脊髓炎大鼠脊髓中Disabled-2 蛋白的免疫组化研究。
Brain Res. 2011 Oct 6;1416:51-60. doi: 10.1016/j.brainres.2011.08.009. Epub 2011 Aug 10.
3
Gene expression in the spinal cord in female lewis rats with experimental autoimmune encephalomyelitis induced with myelin basic protein.实验性自身免疫性脑脊髓炎雌性 Lewis 大鼠脊髓中髓鞘碱性蛋白诱导的基因表达。
PLoS One. 2012;7(11):e48555. doi: 10.1371/journal.pone.0048555. Epub 2012 Nov 6.
4
Immunohistochemical study of flotillin-1 in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis.实验性自身免疫性脑脊髓炎Lewis大鼠脊髓中flotillin-1的免疫组织化学研究
Brain Res. 2006 Oct 9;1114(1):204-11. doi: 10.1016/j.brainres.2006.07.054. Epub 2006 Aug 21.
5
Expression of caveolin-1, -2, and -3 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis.小窝蛋白-1、-2和-3在实验性自身免疫性脑脊髓炎Lewis大鼠脊髓中的表达
J Neuroimmunol. 2005 Aug;165(1-2):11-20. doi: 10.1016/j.jneuroim.2005.03.019.
6
Microglial and astroglial reactions to inflammatory lesions of experimental autoimmune encephalomyelitis in the rat central nervous system.大鼠中枢神经系统中，小胶质细胞和星形胶质细胞对实验性自身免疫性脑脊髓炎炎症性病变的反应。
J Neuroimmunol. 1992 Mar;37(1-2):23-33. doi: 10.1016/0165-5728(92)90152-b.
7
Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.大鼠免疫系统的年龄相关变化：从实验性自身免疫性脑脊髓炎中汲取的经验教训。
Exp Gerontol. 2014 Oct;58:179-97. doi: 10.1016/j.exger.2014.08.005. Epub 2014 Aug 13.
8
Ellagic acid protects from myelin-associated sphingolipid loss in experimental autoimmune encephalomyelitis.鞣花酸可预防实验性自身免疫性脑脊髓炎中的髓鞘相关神经鞘脂丢失。
Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Sep;1863(9):958-967. doi: 10.1016/j.bbalip.2018.05.009. Epub 2018 May 21.
9
Evaluation of a radiolabelled peripheral benzodiazepine receptor ligand in the central nervous system inflammation of experimental autoimmune encephalomyelitis: a possible probe for imaging multiple sclerosis.放射性标记的外周苯二氮䓬受体配体在实验性自身免疫性脑脊髓炎中枢神经系统炎症中的评估：一种用于多发性硬化症成像的潜在探针。
Eur J Nucl Med Mol Imaging. 2005 May;32(5):557-63. doi: 10.1007/s00259-004-1690-y. Epub 2004 Nov 20.
10
The relationship between aquaporin-4 expression and blood-brain and spinal cord barrier permeability following experimental autoimmune encephalomyelitis in the rat.实验性自身免疫性脑脊髓炎大鼠水通道蛋白-4 表达与血脑和血脊髓屏障通透性的关系。
Anat Rec (Hoboken). 2011 Jan;294(1):46-54. doi: 10.1002/ar.21286. Epub 2010 Nov 12.
引用本文的文献
1
Gray matter sodium accumulation and atrophy in relapsing-remitting multiple sclerosis correspond to neurotransmitter maps.复发缓解型多发性硬化症中的灰质钠积累和萎缩与神经递质图谱相对应。
Eur Radiol. 2025 Jul 31. doi: 10.1007/s00330-025-11892-8.
2
Dysregulated Cholinergic Signaling Inhibits Oligodendrocyte Maturation Following Demyelination.胆碱能信号失调抑制脱髓鞘后少突胶质细胞的成熟。
J Neurosci. 2024 Jul 10;44(28):e0051242024. doi: 10.1523/JNEUROSCI.0051-24.2024.
本文引用的文献
1
Cholinergic PET imaging in infections and inflammation using C-donepezil and F-FEOBV.使用碳-多奈哌齐和氟-氟乙基苯乙烯基硼酸酯进行感染和炎症中的胆碱能PET成像。
Eur J Nucl Med Mol Imaging. 2017 Mar;44(3):449-458. doi: 10.1007/s00259-016-3555-6. Epub 2016 Oct 26.
2
Reduced expression of VAChT increases renal fibrosis.囊泡乙酰胆碱转运体表达降低会加重肾纤维化。
Pathophysiology. 2016 Sep;23(3):229-36. doi: 10.1016/j.pathophys.2016.07.002. Epub 2016 Aug 10.
3
Involvement of the Nonneuronal Cholinergic System in Bone Remodeling in Rat Midpalatal Suture after Rapid Maxillary Expansion.非神经元胆碱能系统在大鼠快速上颌扩弓后腭中缝骨重塑中的作用
Biomed Res Int. 2016;2016:8106067. doi: 10.1155/2016/8106067. Epub 2016 Jul 10.
4
Vesicular acetylcholine transporter knock down-mice are more susceptible to inflammation, c-Fos expression and sickness behavior induced by lipopolysaccharide.囊泡型乙酰胆碱转运体敲低小鼠对脂多糖诱导的炎症、c-Fos 表达和疾病行为更为敏感。
Brain Behav Immun. 2016 Oct;57:282-292. doi: 10.1016/j.bbi.2016.05.005. Epub 2016 May 11.
5
PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis.多发性硬化大鼠模型中S1PR1表达的正电子发射断层显像（PET）研究
Mol Imaging Biol. 2016 Oct;18(5):724-32. doi: 10.1007/s11307-016-0944-y.
6
Infiltration of CCR2+Ly6Chigh Proinflammatory Monocytes and Neutrophils into the Central Nervous System Is Modulated by Nicotinic Acetylcholine Receptors in a Model of Multiple Sclerosis.在多发性硬化症模型中，烟碱型乙酰胆碱受体调节CCR2+Ly6Chigh促炎单核细胞和中性粒细胞向中枢神经系统的浸润。
J Immunol. 2016 Mar 1;196(5):2095-108. doi: 10.4049/jimmunol.1501613. Epub 2016 Jan 25.
7
The Role of Astrocytes in Multiple Sclerosis Progression.星形胶质细胞在多发性硬化症进展中的作用。
Front Neurol. 2015 Aug 18;6:180. doi: 10.3389/fneur.2015.00180. eCollection 2015.
8
Nicotinic receptor activation negatively modulates pro-inflammatory cytokine production in multiple sclerosis patients.烟碱受体激活对多发性硬化症患者促炎细胞因子的产生具有负向调节作用。
Int Immunopharmacol. 2015 Nov;29(1):152-7. doi: 10.1016/j.intimp.2015.06.034. Epub 2015 Jul 23.
9
Synthesis and biological characterization of a promising F-18 PET tracer for vesicular acetylcholine transporter.一种有前景的用于囊泡乙酰胆碱转运体的F-18正电子发射断层显像（PET）示踪剂的合成及生物学特性研究
Bioorg Med Chem. 2015 Aug 1;23(15):4699-4709. doi: 10.1016/j.bmc.2015.05.058. Epub 2015 Jun 5.
10
Non-neuronal cholinergic system in regulation of immune function with a focus on α7 nAChRs.非神经元胆碱能系统对免疫功能的调节，重点关注α7烟碱型乙酰胆碱受体。
Int Immunopharmacol. 2015 Nov;29(1):127-34. doi: 10.1016/j.intimp.2015.04.015. Epub 2015 Apr 20.
Zotero 插件