Department of Ophthalmology, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan, Hubei, China (mainland).
Med Sci Monit. 2018 Feb 4;24:706-710. doi: 10.12659/msm.905657.
BACKGROUND Calcium sensing receptor (CaSR) is widely expressed in many tissues of the body, but it is rarely reported to be expressed in the eyes. This research explored the expression and distribution of CaSR in eye tissues of normal and diabetic rats. MATERIAL AND METHODS Thirty male Sprague Dawley (SD) rats were randomly divided into a diabetic and a normal group. Diabetes mellitus (DM) models were successfully established by intraperitoneal injection of streptozotocin. The expression and distribution of CaSR in the rat eyeballs were detected by immunohistochemistry. Quantitative RT-PCR and western blotting were used to detect the presence of CaSR in normal and diabetic rats. RESULTS CaSR was detected in the cornea, lens epithelium, and retina. CaSR was expressed the most in the cornea, followed by the lens epithelium, and the retina (p<0.05). The expression of CaSR was decreased in the eye tissue of diabetic rats (p<0.05). CONCLUSIONS In this study, CaSR was detected in rat cornea, lens, and retina. It was significantly decreased in the eyes of diabetic rats. This indicated that the downregulated expression of CaSR was associated with diabetic oculopathy.
钙敏感受体（CaSR）广泛表达于体内许多组织中，但很少有报道其在眼部表达。本研究旨在探讨 CaSR 在正常和糖尿病大鼠眼组织中的表达和分布。
30 只雄性 Sprague Dawley（SD）大鼠随机分为糖尿病组和正常组。通过腹腔注射链脲佐菌素成功建立糖尿病模型。通过免疫组织化学检测 CaSR 在大鼠眼球中的表达和分布。采用定量 RT-PCR 和 Western blot 检测 CaSR 在正常和糖尿病大鼠中的存在情况。
在角膜、晶状体上皮和视网膜中检测到 CaSR。角膜中 CaSR 的表达最为丰富，其次是晶状体上皮，最后是视网膜（p<0.05）。糖尿病大鼠眼组织中 CaSR 的表达减少（p<0.05）。
本研究在大鼠角膜、晶状体和视网膜中检测到 CaSR。在糖尿病大鼠的眼睛中，CaSR 的表达显著降低。这表明 CaSR 的下调表达与糖尿病性眼病有关。
