Fındıklı Ebru, Camkurt Mehmet Akif, İzci Filiz, Karaaslan Mehmet Fatih, Fındıklı Hüseyin Avni, Sümer Perihan, Kurutaş Ergül Belge
Department of Psychiatry, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Turkey.
Department of Psychiatry, Afşin State Hospital, Afşin, Kahramanmaraş, Turkey.
Clin Psychopharmacol Neurosci. 2018 Feb 28;16(1):88-94. doi: 10.9758/cpn.2018.16.1.88.
Generalized anxiety disorder (GAD) is a common anxiety disorder. Although lots of research done to reveal neurobiological basis of GAD, it is still unclear. Diagnosis of GAD depends on subjective complaints of patients, thus the need for a biological marker is constantly emerging. In this study, we aimed to investigate diagnostic value of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in GAD.
We evaluated MDA, SOD, and CAT levels in peripheral blood of 46 patients and 45 controls. MDA was measured with Ohkawa's methods, SOD was measured with Fridovich method, and CAT was measured with Beutler's method.
MDA was significantly increased in patients than controls, medians 4.05 nmol/mg and 1.71 nmol/mg respectively, ＜0.001; SOD and CAT activity was significantly decreased in patients than controls, medians of SOD were 159.07 U/mg and 301.87 U/mg, ＜0.001 respectively, medians for CAT were 138.47 U/mg and 160.60 U/mg respectively. We found high correlation between Hamilton Anxiety Rating Scale and SOD, MDA r values were 0.723 and 0.715 respectively, ＜0.001 for both. Receiver operator characteristic (ROC) curve analysis showed high diagnostic performance for MDA and SOD, low diagnostic performance for CAT, areas under curve were 1.0, 1.0, and 0.648 respectively.
Our results reveal possible diagnostic value of MDA, less likely of SOD but not CAT. Future studies should investigate diagnostic value of oxidants and antioxidantn enzymes in larger samples and include diagnostic value of these parameters.
广泛性焦虑障碍（GAD）是一种常见的焦虑症。尽管已开展大量研究以揭示GAD的神经生物学基础，但仍不清楚。GAD的诊断依赖于患者的主观主诉，因此对生物标志物的需求不断显现。在本研究中，我们旨在探讨丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在GAD中的诊断价值。
我们评估了46例患者和45例对照者外周血中的MDA、SOD和CAT水平。MDA采用大川法测量，SOD采用弗里多维奇法测量，CAT采用博伊特勒法测量。
患者的MDA水平显著高于对照组，中位数分别为4.05 nmol/mg和1.71 nmol/mg，P＜0.001；患者的SOD和CAT活性显著低于对照组，SOD的中位数分别为159.07 U/mg和301.87 U/mg，P均＜0.001，CAT的中位数分别为138.47 U/mg和160.60 U/mg。我们发现汉密尔顿焦虑量表与SOD、MDA之间高度相关，r值分别为0.723和0.715，两者P均＜0.001。受试者工作特征（ROC）曲线分析显示MDA和SOD具有较高的诊断效能，CAT的诊断效能较低，曲线下面积分别为1.0、1.0和0.648。
我们的结果揭示了MDA可能具有诊断价值，SOD的可能性较小，而CAT不具有诊断价值。未来的研究应在更大样本中研究氧化剂和抗氧化酶的诊断价值，并纳入这些参数的诊断价值。
