Zhao Sha-Sha, Guan Li-Xun, Gao Zhe, Wang Fei-Yan, Wang Li-Li, Gao Chun-Ji
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China; Nankai University School of Medicine, Tianjin 300071,China.
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Feb;26(1):153-158. doi: 10.7534/j.issn.1009-2137.2018.01.026.
To establish the animal model of luciferase-transfected A20 murine B cell lymphoma, so as to provide experimental tools to explore the effect of graft versus tumor.
Luciferase- labeled A20 cells were cloned with puromycin selection. Transfected A20 cells and CBL/6 bone marrow were inoculated into the irradiated BALB/c mice by injection in tailvein to establish the transplantation model. The bioluminescent imaging technique was used to monitor the tumor growth, and then the survival, body weight, tumor formation and pathological characteristics of target organs were observed.
A20 cell line stably expressing luciferase gene was successfully obtained. The the bioluminicent imaging found that the tumor luminescence could be observed on day 8 of A20 cell inoculation, and the mean fluorescent intensity was increased along with the tumor growth. Compared with the BMT group, the survival rate and body weight of BMT+A20-Luc mice were decreased significantly. General anatomy showed the tumor mainly formed in the liver and spleen.
A mouse transplantation model with luciferase- transfected A20 cells has been successfully established, thus laying a foundation for investigation of graft-versus-tumor.
建立荧光素酶转染的A20小鼠B细胞淋巴瘤动物模型，为探讨移植物抗瘤效应提供实验工具。
用嘌呤霉素筛选法克隆荧光素酶标记的A20细胞。将转染后的A20细胞和CBL/6骨髓通过尾静脉注射接种到经照射的BALB/c小鼠体内，建立移植模型。采用生物发光成像技术监测肿瘤生长，然后观察生存情况、体重、肿瘤形成及靶器官的病理特征。
成功获得稳定表达荧光素酶基因的A20细胞系。生物发光成像发现，接种A20细胞第8天可观察到肿瘤发光，且平均荧光强度随肿瘤生长而增加。与骨髓移植（BMT）组相比，BMT+A20-Luc小鼠的生存率和体重显著降低。大体解剖显示肿瘤主要形成于肝脏和脾脏。
成功建立了荧光素酶转染A20细胞的小鼠移植模型，为移植物抗瘤研究奠定了基础。
