Department of Bacteriology, Animal Health Trust, Lanwades Park, Kentford, Newmarket CB8 7UU, UK.
Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, Sweden.
Vaccine. 2018 Mar 7;36(11):1484-1490. doi: 10.1016/j.vaccine.2018.01.030. Epub 2018 Feb 2.
The host-restricted pathogen Streptococcus equi causes strangles in the horse, which is characterised by abscessation of the lymph nodes of the head and neck. The disease is endemic throughout the world causing considerable welfare and economic cost to the horse industry. Here we report the results of three studies where ponies were vaccinated with combinations of recombinant fusion proteins to optimise vaccine production and the level of protection conferred. Optimal protection was conferred by a prototype multicomponent subunit vaccine, Strangvac 4, which contained eight proteins CNE, SclC, SclF, SclI, EAG (fused as CCE), SEQ_402, SEQ_0256 (fused as Eq85) and IdeE. Across the three experiments only three of 16 ponies vaccinated with Strangvac 4 became pyretic compared to all 16 placebo-vaccinated control ponies (P < .001). S. equi was recovered from the lymph nodes of eight Strangvac 4-vaccinated and 15 control ponies (P = .016). None of the ponies vaccinated with Strangvac 4, or the other prototype vaccines developed adverse reactions following vaccination. Our data provide evidence in support of the further clinical development of the Strangvac 4 vaccine.
马疱疹病毒（Streptococcus equi）是一种宿主限制性病原体，可引起马的“腺疫”，其特征是头颈部淋巴结脓肿。该疾病在全球范围内流行，给马业造成了巨大的福利和经济损失。在这里，我们报告了三项研究的结果，这些研究使用重组融合蛋白组合对小马进行了疫苗接种，以优化疫苗生产和保护水平。由八种蛋白 CNE、SclC、SclF、SclI、EAG（融合为 CCE）、SEQ_402、SEQ_0256（融合为 Eq85）和 IdeE 组成的原型多组分亚单位疫苗 Strangvac 4 提供了最佳保护。在这三项实验中，只有 16 匹接种 Strangvac 4 的小马中有 3 匹出现发热，而 16 匹安慰剂接种的对照小马全部出现发热（P<0.001）。在 8 匹接种 Strangvac 4 的小马和 15 匹对照小马的淋巴结中均分离到了 S. equi（P=0.016）。没有一匹接种 Strangvac 4 或其他原型疫苗的小马在接种后出现不良反应。我们的数据提供了证据支持进一步开发 Strangvac 4 疫苗。
