Menon Seema, Lawrence Lincy, Sivaram Vipin P, Padikkala Jose
Amala Cancer Research Centre (Recognized Research Centre of University of Calicut), Amala Nagar, Thrissur 680 555, Kerala, India.
Amala Cancer Research Centre (Recognized Research Centre of University of Calicut), Amala Nagar, Thrissur 680 555, Kerala, India.
J Ayurveda Integr Med. 2019 Jul-Sep;10(3):159-165. doi: 10.1016/j.jaim.2017.06.007. Epub 2018 Feb 3.
Oroxylum indicum Vent., a Dasamula plant used in Ayurveda possesses antioxidant properties.
To evaluate the cardioprotective effect of 70% methanolic extract of O. indicum Vent. root bark (OIM) against doxorubicin induced cardiomyopathy in female Sprague Dawley rats.
Cardiotoxicity was induced by intra-peritoneal injection of doxorubicin 30 mg/kg body weight (b.w.) for 4 consecutive days after a ten-day pre-treatment of animals with OIM at 200 mg/kg b.w. and 400 mg/kg b.w (p.o.). Drug treatment continued up to day 14. Probucol, orally administered at a dose of 20 mg/kg b.w. served as standard. ECG was recorded. The animals were sacrificed on day 15 and comparative analysis of serum marker levels of creatine phosphokinase (CPK), lactate dehydrogenase (LDH), Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), tissue antioxidant status based on Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), reduced Glutathione (GSH) and lipid peroxidation (LPO) was carried out. Histopathological examination was carried out using hematoxylin-eosin staining.
ECG records of OIM treated animals showed normal pattern, in comparison to the control with ST depression and arrhythmia in cardiogram. Tissue antioxidant profile (SOD, GSH and GPx) was significantly (p < 0.01) elevated in the cardiac tissue of treated group in dose-dependent manner; lipid peroxidation level was found to decrease with treatment. Comparative analysis of serum markers - CPK, LDH, SGOT and SGPT - among untreated control, standard and extract treated groups revealed that OIM extract at 400 mg/kg b.w. dose significantly reduced the levels (p < 0.01). Histological analysis revealed normal myocardial architecture in OIM treated groups. HPTLC fingerprint of OIM revealed 8 bands and detected the presence of chrysin, apigenin and quercetin.
O. indicum root bark shows marked cardio-protective activity, possibly due to the presence of antioxidant compounds acting synergistically.
阿育吠陀医学中使用的十大功劳属植物印度木蝴蝶具有抗氧化特性。
评价印度木蝴蝶根皮70%甲醇提取物（OIM）对阿霉素诱导的雌性斯普拉格-道利大鼠心肌病的心脏保护作用。
在以200mg/kg体重和400mg/kg体重（口服）的OIM对动物进行为期10天的预处理后，连续4天腹腔注射阿霉素30mg/kg体重诱导心脏毒性。药物治疗持续至第14天。以口服剂量20mg/kg体重的普罗布考作为标准对照。记录心电图。在第15天处死动物，并对肌酸磷酸激酶（CPK）、乳酸脱氢酶（LDH）、血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）的血清标志物水平、基于超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）的组织抗氧化状态以及脂质过氧化（LPO）进行比较分析。采用苏木精-伊红染色进行组织病理学检查。
与心电图出现ST段压低和心律失常的对照组相比，OIM处理组动物的心电图记录显示为正常模式。处理组心脏组织中的组织抗氧化谱（SOD、GSH和GPx）以剂量依赖方式显著升高（p<0.01）；发现脂质过氧化水平随治疗而降低。对未处理的对照组、标准对照组和提取物处理组之间的血清标志物CPK、LDH、SGOT和SGPT进行比较分析发现，400mg/kg体重剂量的OIM提取物显著降低了这些水平（p<0.01）。组织学分析显示OIM处理组心肌结构正常。OIM的高效薄层色谱指纹图谱显示有8条带，并检测到白杨素、芹菜素和槲皮素的存在。
印度木蝴蝶根皮显示出显著的心脏保护活性，可能是由于抗氧化化合物协同作用的结果。
